Invitrogen™ SYBR™ Gold Nucleic Acid Gel Stain (10,000X Concentrate in DMSO)

Description

Use with UV transilluminators, the Dark Reader, laser scanners, or blue light transilluminators.

• Ultra Sensitive: 25–100 times more sensitive than ethidium bromide. Detect as little as 25pg of DNA
• Improved DNA Cloning Efficiency: Excitable with blue light transillumination, which does not cause DNA damage
• Increased Signal to Background Ratio: More than 1000-fold signal enhancement when bound to nucleic acids
• Versatile: Detects dsDNA, ssDNA and RNA in native, glyoxal, formaldehyde or urea gels

Stain Properties SYBR Gold stain is a proprietary unsymmetrical cyanine dye that exhibits >1000-fold fluorescence enhancement upon binding to nucleic acids and has a high quantum yield (∽0.6) upon binding to double- or single-stranded DNA or to RNA¹. Excitation maxima for dye-nucleic acid complexes are at ∽495nm and ∽300nm and the emission maximum is ∽537 nm.

Stain Sensitivity SYBR Gold stain is >10-fold more sensitive than ethidium bromide for detecting DNA and RNA in denaturing urea, glyoxal, and formaldehyde gels, even with 300nm transillumination. SYBR Gold stain has also been shown to be much more sensitive than SYBR Green II stain for detecting single strand conformation polymorphism (SSCP) products and is much more sensitive than silver-staining for the detection of double-stranded DNA in native polyacrylamide gels.

Agarose Gel Electrophoresis, DNA and RNA Purification and Analysis, Nucleic Acid Gel Electrophoresis and Blotting

¹Tuma RS, Beaudet MP, Jin X, Jones LJ, Cheung CY, Yue S, Singer VL. Characterization of SYBR Gold nucleic acid gel stain: a dye optimized for use with 300-nm ultraviolet transilluminators. Anal Biochem (1999) 268:278-288. (PMID: 10075818)

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