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Invitrogen™ T4 DNA Ligase (5 U/μL)

Catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3' hydroxyl and 5' phosphate termini

Brand:  Invitrogen™ 15224041

653.36 DKK valid until 2025-03-21
Use promo code "24111" to get your promotional price.

Product Code. 10443242

  • 980.00 DKK / 250 units

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To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24111” to get your promotional price


Includes: T4 DNA Ligase is supplied with a vial of 5X reaction buffer [250mM Tris-HCl (pH 7.6), 50mM MgCl2, 5mM ATP, 5mM DTT, 25% (w/v) polyethylene glycol-8000].
This item is not returnable. View return policy

Description

Description

The unique T4 DNA Ligase buffer optimizes ligation, which can be performed in 5 minutes. Single-stranded nucleic acids are not substrates for this enzyme.

Unit Definition: One unit catalyzes the exchange of 1nmol 32P-labeled pyrophosphate into ATP in 20 min. at 37°C. (One unit is equal to approximately 300 cohesive-end ligation units.)

Unit Reaction Conditions: 66mM Tris-HCl (pH 7.6), 6.6mM MgCl2 , 10mM DTT, 66μM ATP, 3.3μM 32P-labeled pyrophosphate, and enzyme in 0.1mL for 20 min. at 37°C.

Order Info

Shipping Condition: Dry Ice

TRUSTED_SUSTAINABILITY
Specifications

Specifications

5 U/μL
Dry Ice
5X Reaction Buffer
T4 DNA Ligase
T4 DNA Ligase is supplied with a vial of 5X reaction buffer [250 mM Tris-HCl (pH 7.6), 50 mM MgCl2 , 5 mM ATP, 5 mM DTT, 25% (w/v) polyethylene glycol-8000]. Store at -20°C.
Ligase
250 U
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Lot Number Certificate Type Date Catalog Number
Lot Number3063173 Certificate TypeCertificate of Analysis Date31/01/2025 Catalog Number15224041
Lot Number2520310 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog Number15224041
Lot Number2612756 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog Number15224041
Lot Number2852593 Certificate TypeCertificate of Analysis Date27/01/2025 Catalog Number15224041
Lot Number2954108 Certificate TypeCertificate of Analysis Date26/01/2025 Catalog Number15224041
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Product Title
Invitrogen™ T4 DNA Ligase (5 U/μL) > 250 units

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