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Invitrogen™ Lipofectamine™ 3000 Transfection Reagent

Lipofectamine™ 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results.

Brand: Invitrogen™ L3000075

31423.64 DKK valid until 2025-12-31
Use promo code "24270" to get your promotional price.

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Product Code. 15222475

43450.00 DKK / 7.50mL

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Descripción

This reagent delivers superior transfection efficiency and improved cell viability for the widest range of hard-to-transfect and common cells (e.g., HEK293, HeLa).

Successfully transfect the widest variety of biologically relevant cell types with a reagent that offers:

Superior performance—our highest efficiency reagent for difficult-to-transfect cells
Improved cell viability—gentle on your cells, with low toxicity
Versatile—one reagent for DNA, RNA, and co-transfection

Efficiently Transfect Difficult-to-Transfect Cells
Lipofectamine 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types. Successfully transfected cell types include:

Fibroblast (3T3, COS-7)
Myoblast (C2C12, L6 CRL-1458)
Hepatocyte (HepG2, HuH7)
Erythroleukemic cell (K562)
Breast carcinoma (MCF7, Hs578T)
Prostate carcinoma (LNCap)
Lung carcinoma (A549, NCI-H460)
Osteosarcoma (U-2 OS, Saos-2)
Colon carcinoma (Caco2, SW480)
Pancreatic carcinoma (PANC-1)
Skin melanoma (SK-MEL-28)

Transfect Common Cells Efficiently and Cost-Effectively
For common cell types, Lipofectamine 3000 reagent is more cost-effective than other reagents due to its high concentration and low usage amount. The 1.5mL size provides up to 1500 transfection reactions (in 24-well plates).

Achieve Superior Transfection Efficiency While Maintaining Low Toxicity
Lipofectamine 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization. A low toxicity lipid dose (0.75μL for 24-well plates) is suggested for applications requiring minimal disruption of the cells.

Enable High Transfection Efficiency in Novel Genome Editing Applications
Lipofectamine 3000 reagent is suitable for novel genome editing applications. It increases the likelihood of successful cleavage and recombination with TALENs or CRISPRs through highly efficient transfection, ultimately maximizing the efficiency of genetic modifications and simplifying the downstream processes.

Especificaciones

Classification	Animal Origin-Free
Cell Type	Established Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells
Sample Type	Plasmid DNA, Synthetic siRNA, RNAi Plasmids (shRNA, miR)
For Use With (Application)	Transfection
Product Line	Lipofectamine™
Quantity	5 x 1.5 mL

Content And Storage	Contains one vial of Lipofectamine™ 3000 Reagent and one vial of P3000TM Enhancer Reagent. Store at 2–8°C. Do not freeze.
Format	6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Transfection Technique	Lipid-based Transfection
Green Features	Sustainable packaging
Product Type	Transfection Reagent
Serum Compatible	Yes

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number3060789	Certificate TypeCertificate of Analysis	Date31/01/2025	Catalog NumberL3000075
Lot Number3060757	Certificate TypeCertificate of Analysis	Date31/01/2025	Catalog NumberL3000075
Lot Number3060757	Certificate TypeCertificate of Analysis	Date31/01/2025	Catalog NumberL3000075
Lot Number3028166A	Certificate TypeCertificate of Analysis	Date31/01/2025	Catalog NumberL3000075
Lot Number2474281	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberL3000075

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Invitrogen™ Lipofectamine™ 3000 Transfection Reagent

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