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Thermo Scientific™ MabCaptureC™ RoboColumn™ Pre-packed Column

Thermo Scientific™ MabCaptureC™ RoboColumn™ are small chromatography columns used for fully automated and parallel chromatographic separations on robotic liquid handling workstations.

4281.77 DKK - 6422.65 DKK

Specifications

Binding Capacity >55 to 75 g IgG/L resin at 10% breakthrough (depending on residence time)
Diameter (Metric) 5 mm
Particle Size 75 ±10 μm
Ligand Type Recombinant C domain of Protein A
Matrix Agarose-based, Epoxide-activated
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Products 2
Product Code Brand Bed Volume Price Quantity & Availability  
Product Code Brand Bed Volume Price Quantity & Availability  
30019462
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Thermo Scientific™
5943662200
200 μL
4281.77 DKK
200µL
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30019264
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Thermo Scientific™
5943662600
600 μL
6422.65 DKK
600µL
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Description

Description

MabCaptureC™ RoboColumn™ are small chromatography columns used for fully automated and parallel chromatographic separations on robotic liquid handling workstations.

These pre-packed columns are ready to connect and have the following features:

  • Packed with MabCaptureC™ Affinity Matrix, offering high dynamic binding capacity and high resolution over a wide range of flow rates
  • Direct connection to standard chromatography systems

The 1 mL columns can be used for quick screenings of application feasibility and lab scale purification on a convenient and easy-to-use pre-packed column. The 10-cm bed height of the 5 mL column allows initial process development at a bench scale.

The pre-packed MabCaptureC™ Affinity Matrix contains engineered rProtein A that binds at the CH2-CH3 interface of the Fc-region of IgGs and shows cross binding to the VH3 domain of IgGs.

Features of MabCaptureC™ Affinity Matrix include:

  • High dynamic mAb binding capacity of > 50 g/L at 4.8 min residence time
  • Purification of mAbs and a subpopulation of scFv's and Fab fragments containing VH3 binding domains
  • Clean and sanitize with standard sodium hydroxide agents for chromatography products
  • Excellent scalability
  • Non-animal derived

Main resin characteristics:

  • Matrix: agarose-based, epoxide activated
  • Average particle size: 75 ± 10 μm
  • Ligand: recombinant C domain of Protein A
  • Ligand coupling method: epoxide coupling
  • Binding capacity: >55 g IgG/L resin at 5 min residence time and 10% breakthrough; >75 g IgG/L resin at 10 min. residence time and 10% breakthrough
  • Elution conditions: 0.1 M glycine pH 3.0, or 20–100 mM sodium citrate or acetate, pH 3.0
  • Cleaning conditions: 0.2 M sodium hydroxide for 15 minutes every cycle
  • Column dimensions: 0.8 x 2 cm (1 mL), 0.8 x 10 cm (5 mL)
  • Operating pressure: ≤ 3 bar
  • Formulation buffer: 20% (v/v) ethanol
Specifications

Specifications

>55 to 75 g IgG/L resin at 10% breakthrough (depending on residence time)
75 ±10 μm
Agarose-based, Epoxide-activated
Agarose bead
8-well strip
5 mm
Recombinant C domain of Protein A
≤3 bar
IgG mAbs, and a subpopulation of scFv and Fab containing VH3 domain
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For Research Use Only. Not for use in diagnostic procedures.