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IBA Lifesciences pCSG-IBA Mammalian Expression Vector with No Secretion
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Designed for high-level episomal expression in mammalian hosts under G418 selection. IBA Lifesciences GmbH Mammalian Expression Vector with No Secretion is a large expression vector with universal features for transient expression as well as for generation of stable cell lines (NeoR).

1985.00 DKK - 2140.00 DKK

Specifications

Constitutive or Inducible System Constitutive
Marker Gene Ampicillin
Promoter CMV
Product Type Vector
Selection Agent (Eukaryotic) Geneticin™ (G-418)
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Products 17
Product Code Brand Vector Price Quantity & Availability  
Product Code Brand Vector Price Quantity & Availability  
15613858
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IBA Lifesciences
5-5003-001
pCSG-IBA3 N/A N/A N/A
15613868
GREENER_CHOICE
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IBA Lifesciences
5-5064-001
pCSG-IBA64 N/A N/A N/A
15613878
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IBA Lifesciences
5-5145-001
pCSG-IBA145 N/A N/A N/A
15623858
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IBA Lifesciences
5-5005-001
pCSG-IBA5 N/A N/A N/A
15623878
GREENER_CHOICE
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IBA Lifesciences
5-5162-001
pCSG-IBA162 N/A N/A N/A
15633858
GREENER_CHOICE
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IBA Lifesciences
5-5023-001
pCSG-IBA23 N/A N/A N/A
15633878
GREENER_CHOICE
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IBA Lifesciences
5-5164-001
pCSG-IBA164 N/A N/A N/A
15643868
GREENER_CHOICE
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IBA Lifesciences
5-5103-001
pCSG-IBA103
2140.00 DKK
5µg
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15643878
GREENER_CHOICE
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IBA Lifesciences
5-5167-001
pCSG-IBA167 N/A N/A N/A
15653878
GREENER_CHOICE
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IBA Lifesciences
5-5168-001
pCSG-IBA168 N/A N/A N/A
15663868
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IBA Lifesciences
5-5105-001
pCSG-IBA105
2140.00 DKK
5µg
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15673858
GREENER_CHOICE
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IBA Lifesciences
5-5043-001
pCSG-IBA43 N/A N/A N/A
15673868
GREENER_CHOICE
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IBA Lifesciences
5-5123-001
pCSG-IBA123 N/A N/A N/A
15683858
GREENER_CHOICE
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IBA Lifesciences
5-5045-001
pCSG-IBA45 N/A N/A N/A
15693848
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IBA Lifesciences
5-5000-001
pCSG-IBAwt1
2140.00 DKK
5µg
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15693858
GREENER_CHOICE
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IBA Lifesciences
5-5062-001
pCSG-IBA62 N/A N/A N/A
15693868
GREENER_CHOICE
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IBA Lifesciences
5-5143-001
pCSG-IBA143 N/A N/A N/A
Descrizione

Descrizione

pCSG-IBA mammalian expression vectors

  • Designed for high-level episomal expression in mammalian hosts under G418 selection containing the following elements:
    • Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
    • Episomal replication through Epstein Barr Virus replication origin (oriP) and nuclear antigen encoded by EBNA-1 in human, primate and canine cells and through SV40 replication origin in cells latently infected with SV40 or that express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7)
  • Stable cell lines can be selected by the neomycin resistance gene (NeoR).
  • In addition, the human cytomegalovirus (CMV) immediate-early promoter enables a high-level expression in a wide range of mammalian cells.

Please note that cloning into pCSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. Besides to the direct cloning of the gene of interest into pCSG-IBA vectors with Esp3I, another option via a so-called entry-vector is possible.

Specifica

Specifica

Constitutive
CMV
Geneticin™ (G-418)
Direct Cloning
Forward
Episomal expression (oriP, EBNA-1)
Ampicillin
Vector
Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage
250 ng/μL
5 μg
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IBA Lifesciences pCSG-IBA Mammalian Expression Vector with No Secretion

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