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Invitrogen™ mMESSAGE mMACHINE™ T3 Transcription Kit

Obtain 10–50 times yield of conventional in vitro transcription reactions

6210.00 DKK


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Product Code Brand Product Type No. of Reactions Price Quantity & Availability  
Product Code Brand Product Type No. of Reactions Price Quantity & Availability  
10665605
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Invitrogen™
AM1348
T3 In Vitro Transcription kit 25 Reactions
6210.00 DKK
25 reactions
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Description

Description

Ambion mMESSAGE mMACHINE High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15 - 35μg of 7-methyl guanosine capped RNA from 1μg of template DNA. Each kit includes sufficient reagents for 25 reactions.

  • High yields of capped RNA—up to 39μg of RNA per reaction
  • Extra convenience—cap analog-nucleotide mix provided
  • Simplified reaction format
  • Synthesized transcripts ideal for microinjection, in vitro translation, or transfection in a 20μL reaction during a 2 hour incubation, mMESSAGE mMACHINE High Yield Capped RNA Transcription will yield up to 35μg of capped RNA
  • 10–50 times yield obtained with conventional in vitro transcription reactions
  • Ratio of cap analog to GTP has been optimized to allow compromise between yield (15 - 35μg) and proportion of transcripts that are capped (80%)
  • Contain a LiCl precipitation solution, efficient for separating proteins and unincorporated nucleotides (including free cap analog) from the capped RNA, allowing an increased efficiency of translation
  • Optimized for use with polymerases included in kit
  • Contains 57U of cap analog

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For Research Use Only. Not for use in diagnostic procedures.