Non-radioactive technique
Brand: Invitrogen™ C10289
The Click-iT AHA Alexa Fluor 488 Protein Synthesis HCS Assay provides a fast, sensitive, non-toxic and non-radioactive method for the detection of nascent protein synthesis utilizing fluorescence microscopy and high-content imaging. Click-iT AHA is an amino acid analog of methionine containing an azido moiety. Similar to 35S-methionine, Click-iT AHA is fed to cultured cells and incorporated into proteins during active protein synthesis. Detection of the incorporated amine acid utilizes a chemoselectiove ligation or 'click' reaction between an azide and an alkyne, where the azido modified protein is detected with the green-fluorescent Alexa Fluor 488 alkyne. The Click-iT AHA Alexa Fluor 488 Protein Synthesis HCS Assay has been successfully tested in A549 and U-2 OS cells with a variety of reagents that inhibit protein synthesis including cycloheximide, anisomycin and puromycin in both dose response and Min/Max format.
Cell Analysis, Cell Metabolism, Cell Viability and Cytotoxicity, Cell Viability, Proliferation and Function
Shipping Condition: Room temperature
| Blue, Green | |
| Fluorescence | |
| Fluorescence Microscope, High Content Analysis Instrument | |
| Alexa Fluor™ 488, Hoechst 33258 | |
| Less hazardous | |
| 2 plates |
| The kit contains sufficient reagents for performing 2 plates in a 96-well plate format. |
|
| High-content Screening (HCS) | |
| HCS Assay | |
| 96-well plate | |
| Alexa Fluor, Click-iT, Molecular Probes | |
| Room Temperature |
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For Research Use Only. Not for use in diagnostic procedures.
