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Invitrogen™ No-Stain™ Protein Labeling Reagent
A fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that provides sensitive, linear detection of protein for total protein normalization of western blotting data. The Invitrogen No-Stain Reagent can also be used as a fast, sensitive gel stain for visualization of proteins in a gel.
414.00 DKK - 1238.00 DKK
Especificaciones
Description | The No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins |
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Membrane Compatibility | PVDF, Nitrocellulose |
Product Line | Invitrogen™ |
For Use With (Equipment) | Compatible with wide-range of imagers with UV or Fluorescence light sources, for example, the iBright imager |
Run Time | 10 minutes |
Código de producto | Marca | No. of Reactions | Gel Compatibility | Precio | Cantidad y disponibilidad | ||||
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Código de producto | Marca | No. of Reactions | Gel Compatibility | Precio | Cantidad y disponibilidad | ||||
16351358
|
Invitrogen™
A44717 |
A standard kit will label 10 mini gels or 10 mini gel-sized membranes | Mini Precast Protein Gels, Midi Precast Protein Gels |
414.00 DKK
10 reactions |
In Stock
Log in to see stock. |
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16341358
|
Invitrogen™
A44449 |
A standard kit will label 40 mini gels or 40 mini gel-sized membranes | Any Gel Type and Gel Chemistry |
1238.00 DKK
40 reactions |
In Stock
Log in to see stock. |
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Descripción
- Flexible use labeling format—Use with any protein gel of choice to perform total protein labeling of the membrane post-transfer. Or use it as a fast protein stain after electrophoresis of gels you do not intend to transfer.
- Easy to use protocol—Mix and incubate with the transferred membrane. The reaction time is 10 minutes. Gel staining is also a 10 minute reaction.
- Flexible visualization—Use a wide-range of imagers with UV, Green LED or Fluorescence (∼488 mm) light source, including the iBright FL1500 Imaging System, to capture an image.
- Accurate total protein normalization—Designed to provide accurate total protein normalization over a broad linear range for protein detection: 1–80 μg total protein loaded per well.
- Sensitive and stable signal—Protein bands are detected down to 20 ng and the signal is compatible with downstream antibody detection.
- Total protein normalization is a useful method for obtaining accurate, quantitative western blotting data, as housekeeping proteins can often be affected by experimental conditions.
Especificaciones
The No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins | |
Invitrogen™ | |
10 minutes | |
Protein Labeling Reagent |
PVDF, Nitrocellulose | |
Compatible with wide-range of imagers with UV or Fluorescence light sources, for example, the iBright imager | |
Dry Ice | |
Compatible with all downstream immunodetection steps (blocking, antibody binding, chemiluminescent or fluorescence based detection |
Seguridad y manipulación
missing translation for 'shelfLife' : At least 2 years from receipt
For Research Use Only. Not for use in diagnostic procedures.
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