Normal Rabbit Serum, Invitrogen™

Description

For blocking purposes, use a 5% (v/v) solution (1:20 if reconstituted at the recommended volume). Reconstitute using 2 mL distilled water and centrifuge if product is not completely clear after standing for 1-2 hrs at room temperature. Once reconstituted, the product may be stored for up to six weeks at 4oC. Do not use for more than one day after diluting the product. For long term storage, aliquote and store at -20oC, avoiding freeze/thaw cycles. Country of Origin: USA

Normal Sera are non-immune serum samples from goat, human, mouse, rabbit and other species that contain the usual complement of serum proteins, including the various classes of immunoglobulins that exist in healthy animals of the particular species. Normal serum provides a sufficient quantity of endogenous proteins to saturate and block nonspecific binding sites on fixed samples or assay substrates. When used as an antibody diluent, non-immune serum also supplies a native chemical environment for antibodies to function properly without adding foreign protein components. Normal serum is frequently used for blocking or saturating generalized binding interactions for immunodetection methods, especially those involving tissue samples such as immunohistochemistry (IHC). Normal sera are also useful as controls for testing general and specific antibody purification methods.