Invitrogen™ Novex™ Midi Tris-Glycine Plus Resupply Pack, 10%

Description

The Novex Tris-Glycine Resupply Pack simplifies your protein separation workflow. This pack, sufficient for 100 gel runs, includes precast midi gels, running buffer, and protein ladders. Novex Tris-Glycine gels provide reproducible separation of a wide range of proteins into well-resolved bands, based on traditional Laemmli protein electrophoresis. They offer flexibility for both native and denaturing protein gel electrophoresis. With a long shelf life and the innovative WedgeWell format that allows for easier sample loading, this pack is a reliable and convenient solution for your laboratory needs.

The Novex Tris-Glycine Resupply Pack contains:

• Novex Tris-Glycine gels (10 boxes, 100 gels)
• Novex Tris-Glycine Running Buffer, 10X (5 L, Cat. No. LC26755)
• PageRuler Plus Pre-stained Protein Ladder, 10 to 250 kDa (Cat. No. 26619)

About Novex Tris-Glycine gels

Invitrogen Novex Tris-Glycine Gels are based on traditional Laemmli protein electrophoresis, allowing the use of Laemmli sample and running buffers. These precast polyacrylamide gels are designed to provide reproducible separation of a wide range of proteins into well-resolved bands. Novex Tris-Glycine Gels do not contain SDS, enabling you to run your proteins in native or denatured form, offering flexibility for both types of protein gel electrophoresis.

Key features of Novex Tris-Glycine Gels include a long shelf life a fast separation of your proteins using constant voltage in less than 60 minutes. The gels use a tris-glycine discontinuous buffer system, involving three primary ions: Chloride, Glycine, and Tris base, which collectively establish an operating pH of 9.5 in the separating region of the gel. This design ensures well-resolved, straight bands with optimal band quality compared to other commercially available precast gels, thus fulfilling your protein separation needs with superior performance.

Separation

For running Invitrogen protein gels, you have the flexibility to use either the SureLock Tandem Midi Gel Tank (STM1001) or the XCell4 SureLock Midi-Cell (WR0100). Both systems are designed to provide optimal performance. The SureLock Tandem offers rapid and efficient electrophoresis of midi gels in a leak-free system, with minimal buffer usage. The two independent chambers allow for electrophoresis of one or two gels simultaneously, conserving buffer and reducing waste. Alternatively, the XCell4 SureLock Midi-Cell is a robust and efficient system for midi-gel electrophoresis. Its standout feature is its capacity to simultaneously run up to four midi gels, enabling high-throughput protein analysis with enhanced efficiency and convenience. Additionally, the SureLock Tandem Midi Gel Tank is conveniently available through Invitrogen Precast Gel Welcome Packs along with both sample buffer and reducing agent, providing an all-in-one solution for your electrophoresis needs.

Transfer

For transferring your proteins to a membrane, we recommend using for Novex Tris-Glycine Native Running Buffer (LC2672) or Novex Tris-Glycine Transfer Buffer (LC3675) for traditional wet transfer using the SureLock Tandem Midi Blot Module (STM2001). Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 3 Gel Transfer Device (IB31001).