missing translation for 'onlineSavingsMsg'
Learn More
Learn More
OPTIZYME™ XbaI, Fisher BioReagents™
575.00 DKK - 1645.00 DKK
Specifications
| Components | 10X OPTIZYME™ Buffer 4 |
|---|---|
| Incubator Temperature | 37°C |
| pH | 7.4 |
| For Use With (Application) | Indicates ligation efficiency of DNA fragments generated by digestion with the Restriction Enzyme |
| Storage Buffer | 10mM Tris HCl (pH 7.4), 100mM KCl, 7mM 2-mercaptoethano, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol |
| Product Code | Brand | Concentration | Quantity | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | Concentration | Quantity | Price | Quantity & Availability | |||||
|
11994842
|
Fisher BioReagents
BP8008-5 |
50 U/μL | 10,000 U | N/A | ||||||
|
11984842
|
Fisher BioReagents
BP8008-1 |
10 U/μL | 2000 U |
575.00 DKK
Each |
Please sign in to purchase this item. Need a web account? Register with us today! | |||||
Description
5'...T^C T A G A...3'
3'...A G A T C^T...5'
Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity
- 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
- Incubate at 37°C
- Buffer 1: 50 - 100%
- Buffer 2: 50 - 100%
- Buffer 3: 20 - 50%
- Buffer 4: 100%
- Buffer 5: 0 - 20%
10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 7mM 2-mercaptoethanol, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
Ligation and Re-cleavage:
More than 95% of the DNA fragments can be ligated and re-cut after a 50-fold over-digestion with XbaI.
Digestion of Agarose-embedded DNA:
A minimum 5 units of XbaI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
Notes:
- XbaI is blocked by overlapping dam methylation.
- To avoid dam methylation, use a dam-, dcm- strain.
Methylation Effects:
Dam: May overlap - blocked
- 5'...TCTAGm6A TC...3'
- 3'...AGATC Tm6AG...5'
- (Cleavage is blocked)
CpG: Never overlaps - no effect
EcoKI: Never overlaps - no effect
EcoBI: Never overlaps - no effect
Specifications
| 10X OPTIZYME™ Buffer 4 | |
| 7.4 | |
| 10mM Tris HCl (pH 7.4), 100mM KCl, 7mM 2-mercaptoethano, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol | |
| XbaI |
| 37°C | |
| Indicates ligation efficiency of DNA fragments generated by digestion with the Restriction Enzyme | |
| T.CTAGA | |
| Liquid |
Safety and Handling
Product Identifier
- OPTIZYME
Spot an opportunity for improvement?Share a Content Correction
Product Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title
OPTIZYME™ XbaI, Fisher BioReagents™