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Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with HF or GC Buffer

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

1646.00 DKK - 6510.00 DKK

Specifications

Polymerase Phusion High-Fidelity DNA Polymerase
Reaction Speed Fast
Product Type High-Fidelity PCR Master Mix
Shipping Condition Dry Ice
For Use With (Application) Standard PCR, High-fidelity PCR
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Products 4
Product Code Brand Includes No. of Reactions Price Quantity & Availability  
Product Code Brand Includes No. of Reactions Price Quantity & Availability  
10523288
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Thermo Scientific™
F532S
GC Buffer 100 Reactions
1648.00 DKK
100 reactions
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10094387
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Thermo Scientific™
F532L
GC Buffer 500 Reactions
6510.00 DKK
500 reactions
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10402678
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Thermo Scientific™
F531L
HF Buffer 500 Reactions
6510.00 DKK
500 reactions
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10369537
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Thermo Scientific™
F531S
HF Buffer 100 Reactions
1646.00 DKK
100 reactions
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Description

Description

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.

Phusion High-Fidelity PCR Master Mix is convenient 2X mix containing Phusion DNA Polymerase, nucleotides, and optimized reaction buffer including MgCl2. Two master mix formulations are available: with HF Buffer (F-531S and F-531L) and with GC Buffer (F-532S and F-532L). The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10-7) is lower than that in GC Buffer (9.5 x 10-7). Therefore, the master mix with HF Buffer should be used as the default master mix for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures.

Highlights

  • High fidelity (25X Taq)
  • Fast PCR due to short extension times (15–30 s/kb)
  • Robust performance, minimal optimization needed

Applications

  • Amplification of difficult (GC-rich) templates
  • High-fidelity PCR
  • Cloning
  • Template generation for sequencing
  • Long-range PCR (up to 20 kb)
  • Mutagenesis
  • High throughput PCR

Using Phusion DNA polymerases

Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).

Specifications

Specifications

Phusion High-Fidelity DNA Polymerase
High-Fidelity PCR Master Mix
Standard PCR, High-fidelity PCR
52X
Blunt
20 kb or less
Fast
Dry Ice
2X
No
SuperMix or Master Mix
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Product Certifications

For Research Use Only. Not for use in diagnostic procedures.