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Thermo Scientific™ Pierce™ Biotin Quantitation Kit Product Code.: 10341634
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Thermo Scientific™ Pierce™ Biotin Quantitation Kit

Product Code. 10341634 Shop All Thermo Scientific Products
12 Tests, 1 set
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Quantity:
12 Tests
Unit Size:
1 set
This item is not returnable. View return policy

Product Code. 10341634

Brand: Thermo Scientific™ 28005

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2270.50 DKK valid until 2025-08-31
Use promo code "24157" to get your promotional price.
This item is not returnable. View return policy

Description

HABA dye reagent and biotinylated protein standard for convenient colorimetric determination of biotinylation levels in antibodies and other proteins.

Thermo Scientific™ Pierce Biotin Quantitation Kit includes aliquots of HABA reagent and biotinylated protein standard for convenient colorimetric determination of biotinylation levels in labeled antibodies and other proteins.

Determine the molar ratio of biotin incorporated into a protein using the HABA-avidin method. The HABA dye (4'-hydroxyazobenzene-2-carboxylic acid) binds to avidin to produce a yellow-orange colored complex that absorbs at 500nm. Free biotin present in solution with this avidin-HABA complex will displace the HABA dye and cause the absorbance to decrease.The amount of biotin present can be calculated directly from the decreased absorbance at 500nm. The Thermo Scientific EZ Biotin Quantitation Kit contains pre-measured doses of the Avidin-HABA mixture and biotinylated horseradish peroxidase positive control to simplify reagent preparation and minimize the amount of waste generated. The convenient assay can be performed either in a cuvette or in a microplate.

Highlights:

  • HABA-avidin complex can be used over a wide range of pH and salt concentrations
  • Amount of biotin can be calculated directly from the decreased absorbance at 500nm complexing with the HABA dye

Eliminate the need for cumbersome assay equations with a convenient HABA calculator found at www.piercenet.com/HABA/habacalc.cfm

Includes:

HABA/Avidin Premix, 24 tubes; Biotinylated HRP, 12 tubes

TRUSTED_SUSTAINABILITY

Specifications

Product Type Biotin Quantitation Kit
Quantity 12 Tests
Content And Storage Sufficient For: Testing biotinylation of 12 protein samples
• HABA-Avidin Premix, 24 tubes
• Biotinylated Horseradish Peroxidase, 5 mg

Store at 4°C.
Product Line Pierce™
Label or Dye Biotin
Labeling Target Antibodies, Proteins

Certificates

Certificates

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5 results found
Lot Number Certificate Type Date Product Code
Lot NumberZJ399540A Certificate TypeCertificate of Analysis Date30/10/2024 Product Code28005
Lot NumberZJ399540 Certificate TypeCertificate of Analysis Date30/10/2024 Product Code28005
Lot NumberZJ394395A Certificate TypeCertificate of Analysis Date15/10/2024 Product Code28005
Lot NumberZJ394395 Certificate TypeCertificate of Analysis Date15/10/2024 Product Code28005
Lot NumberZG390745A Certificate TypeCertificate of Analysis Date07/08/2024 Product Code28005

Promotions

  • Multiple Manufacturers

    Big discounts are available on a range of popular products including chemicals, lab essentials, equipment, consumables, life science and safety products.
    Promo Code: 24157

    Expires:  2025-08-31

    Hot Deal

    *Discount will apply to qualifying orders received by Fisher Scientific between 01/07/2025 and 31/08/2025. Promotion code must be presented at the time of order. Discount applies to the list price in effect at the time the order is received. Promotion cannot be combined with other discounts or promotions. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.

    Terms & Conditions See Full Promo Details

For Research Use Only. Not for use in diagnostic procedures.

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