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Thermo Scientific™ Pierce™ Cypridina-Firefly Luciferase Dual Assay Kit Product Code.: 12201662

Thermo Scientific™ Pierce™ Cypridina-Firefly Luciferase Dual Assay Kit

Product Code. 12201662 Shop All Thermo Scientific Products
100 Reaction Kit, Each
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Quantity:
100 Reaction Kit
1000 Reaction Kit
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Each
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Product Code. 12201662

Brand: Thermo Scientific™ 16183

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Simultaneously detect intracellular Cypridina and Red Firefly luciferase activity in mammalian whole cell lysates.

The Thermo Scientific™ Pierce Cypridina-Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular Cypridina and Red Firefly luciferase activity in mammalian whole cell lysates.

The Pierce Cypridina-Firefly Luciferase Dual Assay Kit is a highly sensitive assay that allows for the simultaneous detection of Cypridina and Red Firefly luciferase activity. Cypridina luciferase acts as an experimental reporter with constitutively active red firefly as a normalization control. This reporter-and-control combination enables simultaneous monitoring of experimental reporter and control luciferase activities in a single-read assay without the need for two-step addition of substrate reagents or quenching. The assay working solution contains substrates for both luciferases, and the reactions occur simultaneously with flash-type kinetics. The resulting luminescent signals are spectrally resolvable using filters. In a single sample, researchers can assay transcriptional activity of regulatory elements, signal transduction pathways, and effects of activators or inhibitors.

Highlights:

  • Simultaneous – concurrent, filter-based, wavelength-separated detection of two luciferase activities
  • Sensitive – measure bright blue Cypridina and red firefly luciferase activities in the same sample
  • Fast – no quenching step required, unlike in traditional sequential dual assays
  • Multiplex – capable of quantitating two cellular activities in the same sample(s)

Includes:

Cell lysis buffer, reaction buffer, and substrates Vargulin and D-luciferin

Requires:

Cypridina and Red Firefly luciferase reporters; Filter sets in the ranges of 425 to 525nm for Cypridina Luc and 615 to 675nm for Red Firefly Luc. Luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers; Injector required for assessing more than 24 wells at a time.

Recommended for:

Study two regulatory elements at the same time; Monitor two signaling pathways simultaneously; Enable studying more than one target per screen (e.g. off-target effects)

TRUSTED_SUSTAINABILITY

Specifications

Type Assay Kit
Target Luciferase, Cypridina Luciferase
Sufficient For 100 Dual Luciferase Assays
Quantity 100 Reaction Kit
Content And Storage Sufficient For: 100 dual-luciferase assays in microplate wells
• Cypridina-Firefly Dual Assay Buffer, 5 mL (store at 4°C)
• 100X Vargulin, 50 μL (store at -20°C)
• D-Luciferin, 3 mg (store at 4°C)
• Luciferase Cell Lysis Buffer (2X), 6 mL (store at room temperature)

Upon receipt, store kit at -20°C, or store individual components as indicated above.
Assay Reporter Enzyme, Luciferase Reporter Assay
Compatible Cells Mammalian Cells
Detection Method Bioluminescence
For Use With (Equipment) Luminometer (Microplate)
Format 384-well plate, 96-well plate
Label Type Enzyme Labeled
Substrate Properties Chemical Substrate
Technique Enhanced Chemiluminescence
Substrate Type Luciferase Substrate
Product Line Pierce™
Substrate Vargulin (Cypridina Luciferin), D-Luciferin
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For Research Use Only. Not for use in diagnostic procedures.

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