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Thermo Scientific™ Pierce™ GST Protein Interaction Pull-Down Kit
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Thermo Fisher Scientific Pierce GST Tag Protein Interaction Pull-Down Kits contains the necessary components to capture and purify proteins that interact with GST-tagged fusion proteins.
Features of GST Tag Protein Interaction Pull-Down Kit:
- GST pull-down (Product No. 21516)—gently purifies protein interactors of any GST-tagged fusion protein without denaturing
- Complete kits—provide all components and detailed protocol for purifying protein:protein interactions
- No special equipment needed—use common laboratory equipment and reagents (e.g., microcentrifuge)
- Convenient—microcentrifuge spin columns facilitate simple and efficient manipulation of agarose beads, including simple processing of multiple samples
- Flexible—instructions include protocols for use with bait and prey proteins from many different sources
- Binding—Binds approx. 8 mg of GST-tagged fusion protein per mL of resin
Applications:
- Discover a new protein:protein interaction from a cell lysate
- Confirm a putative interaction from a cell lysate or with a previously purified protein
- Extract protein:protein interaction information from in vitro transcription/translation lysates
You provide the tagged fusion protein as the 'bait' and the cells expressing the putative protein interaction target ('prey'), and the Pull-Down Kits provide everything else: cell lysis buffer, microcentrifuge spin columns, tag-specific affinity resin (agarose beads) and optimized buffers and protocol. The Pull-Down Kits are designed to teach the method to first-time users and to increase ease-of-use, convenience and reproducibility for experienced researchers.
Related Products
Pierce™ His Protein Interaction Pull-Down Kit (Catalog #21277)
Specifications
Specifications
| Bed Volume | 750 ÎĽL |
| Capacity (Metric) | 1.5 mL |
| Formulation | Slurry: 50 % |
| Content And Storage | Sufficient For: At least 25 pull-down reactions, each using 25 μL of resin • Glutathione Agarose, 750 μL • Pull-Down Lysis Buffer, 250 mL • Glutathione, 1 g • TBS Pack (makes 500 mL), 1 pack • Pierce Spin Columns and Accessories, 27 columns • Microcentrifuge Collection Tubes, 100 tubes Upon receipt store Pierce Lysis Buffer at room temperature. Store remaining kit components at 4°C. |
| Assay | Affinity Purification |
| For Use With (Equipment) | Microcentrifuge |
| Product Line | Pierce™ |
| Quantity | 25 reactions kit |
| For Use With (Application) | Pull-Down (Tag-Based) |
| Target | Not Target-Specific |
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Documents
Product Information
Manuals
For Research Use Only. Not for use in diagnostic procedures.
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