Thermo Scientific™ Pierce™ High Capacity NeutrAvidin™ Agarose

NeutrAvidin Resins are prepared by covalently coupling NeutrAvidin Protein to the beaded agarose or UltraLink Biosupport using efficient and stable chemistries, resulting in NeutrAvidin Agarose and UltraLink Resins that are leach-resistant and stable at pH 2-11. The products are excellent choices for a variety of small- or large-scale affinity purification applications involving biotinylated macromolecules, including separation of biotinylated molecules from samples and immunoprecipitation of antigens using biotin-labeled antibodies.

Highlights:

• Low nonspecific binding – unlike avidin, NeutrAvidin Protein has no carbohydrates, eliminating nonspecific binding problems caused by sugars; unlike streptavidin, NeutrAvidin Protein has no Arg-Tyr-Asp sequence, which can be a source of binding to cell surface molecules

• Versatile resins – agarose (polysaccharide-based) and UltraLink Biosupport (acrylamide-based) resins provide two different options for particular experimental conditions and are compatible with gravity-flow, spin and FPLC methods

• Available in multiple formats – choose from two resin types, regular or high capacity preparations, and small or large-scale package sizes (bulk sizes are also available)

• Superior performance – High Capacity Resin has higher binding capacity than best alternative supplier streptavidin products, allowing use of small amounts of resin for experiments

Item-Specific Features:

• Agarose Resins are crosslinked 6% beaded agarose
• UltraLink Resins are beaded acrylamide support (UltraLink Biosupport)
• Plus and High Capacity Resins have a higher loading of NeutrAvidin Protein than the regular forms

Recommended for:

Immunoprecipitation; Purifying proteins that bind to biotinylated ligands; Capturing biotinylated cell-surface proteins; Purifying biotinylated peptides