Thermo Scientific™ Pierce™ Protein A Plus UltraLink™ Resin

Description

Thermo Scientific™ Pierce Protein A Plus UltraLink Resin is an extremely durable beaded polyacrylamide support to which IgG-binding Protein A has been immobilized at high density for use in IgG purification methods.

The Protein A Plus UltraLink Resin is used for high-capacity binding and purification of IgG in gravity-flow, spin-column or medium-pressure chromatography methods. Protein A is a popular antibody-binding protein that is especially useful for purification of IgG from rabbit or human serum.

UltraLink Resin is an azlactone-activated polyacrylamide support that is hydrophilic, charge-free, high capacity, highly crosslinked, rigid and porous. This support is especially useful for medium pressure techniques when using large sample volumes requiring fast-flow techniques (FPLC) and large-scale applications. Unlike typical beaded agarose supports, UltraLink Support does not crush or collapse when subjected to high flow rates that result in pressures exceeding 25psi.

Highlights:

• Immobilized Protein A - specifically binds IgG from several mammalian species, especially most subtypes of human and rabbit IgG

• High capacity - “Plus” variety has a dense load of immobilized Protein A, providing a binding capacity of at least 30mg human IgG per milliliter of resin

• UltraLink Resin - acrylamide-based support provides an alternative to crosslinked beaded agarose supports for medium pressure applications

Properties of Thermo Scientific UltraLink Resin:

• Support pH Stability: 1 to 13
• Average Particle Size: 50 to 80 microns
• Exclusion Limit: greater than 2,000,000 daltons
• Average Surface Area: greater than 250m2 per gram of beads
• Average Pore Volume: greater than 1.2mL per gram of beads (> 60% of bead volume)
• Pore Size: 500 to 1000 angstroms (50 to 100nm)
• Maximum Linear Velocity: 3000cm per hour
• Maximum Pressure: 100psi (6.9 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather than the pressure drop across the column.)