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Thermo Scientific™ Metal Enhanced DAB Substrate Kit
Special formulation of diaminobenzidine peroxidase substrate that uses cobalt and nickel chloride to yield intense color for IHC staining.
Brand: Thermo Scientific™ 34065
This item is not returnable.
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Description
Thermo Scientific™ Pierce Metal-Enhanced DAB Substrate Kit uses cobalt and nickel chloride in a special formulation of diaminobenzidine peroxidase substrate that yields intense color for immunohistochemical staining.
The color produced by DAB (3,3'-diaminobenzidine) can be intensified by the addition of metals such as nickel, copper, silver and cobalt that form more dense complexes upon reaction in HRP-peroxide substrate reactions. The near-black signal (precipitate) is easy to photograph.Highlights:
Incredible sensitivity – 50 times more sensitive than the traditional DAB method and 30 times more sensitive than other metal-intensified versions of DAB
Low background, high intensity – get a crisp dark brown-black precipitate and, even with the increased intensity, background is almost nonexistent
Six-hour stability – working solution is stable for more than six hours at room temperature
Includes:
Metal Enhanced DAB (10X), 25mL; Stable Peroxide Buffer, 250mL
Specifications
| Chromogenic, Colorimetric | |
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| HRP (Horseradish Peroxidase) Substrate | |
| Immunodetection, IHC (Immunohistochemistry) |
| 275 mL of 1X working solution | |
| DAB (Enhanced) | |
| Pierce™ | |
| DAB Substrate Kit |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Metal Enhanced DAB Substrate Kit
Signal Word
- Danger
Hazard Category
- Flammable liquid Category 3
- Acute toxicity Category 3
- Skin corrosion/irritation Category 1 A
- Serious eye damage/eye irritation Category 1
- Respiratory sensitiser Category 1
- Skin sensitiser Category 1
- Carcinogenicity Category 1A
- Reproductive toxicity Category 1B
- Specific target organ toxicity after single exposure Category 1
- Specific target organ toxicity after repeated exposure Category 2
- LONG-TERM AQUATIC HAZARD Chronic 2
Hazard Statement
- H226-Flammable liquid and vapour.
- H301-Toxic if swallowed.
- H311-Toxic in contact with skin.
- H331-Toxic if inhaled.
- H314-Causes severe skin burns and eye damage.
- H318-Causes serious eye damage.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H317-May cause an allergic skin reaction.
- H350-May cause cancer.
- H360FD-May damage fertility. May damage the unborn child.
- H370-Causes damage to organs.
- H373-May cause damage to organs.
- H411-Toxic to aquatic life with long lasting effects.
Precautionary Statement
- P201-Obtain special instructions before use.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P273-Avoid release to the environment.
- P235-Keep cool.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P331-Do NOT induce vomiting.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305-IF IN EYES:.
- P310-Immediately call a POISON CENTER/doctor/.
- P501-Dispose of contents/container to .
Supplemental information
- MIXTURE LIST-Contains : methanol, ethanediol, N-carboxymethyliminobis(ethylenenitrilo)tetra(acetic acid), Cobalt chloride(Co
For Research Use Only. Not for use in diagnostic procedures.