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Thermo Scientific™ EZ-Link™ Plus Activated Peroxidase Product Code.: 11849150
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Thermo Scientific™ EZ-Link™ Plus Activated Peroxidase

Product Code. 11849150 Shop All Thermo Scientific Products
5 x 1mg, Pack of 5
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Quantity:
1 mg
5 x 1mg
Unit Size:
1mg
Pack of 5
This item is not returnable. View return policy

Product Code. 11849150

Brand: Thermo Scientific™ 31488

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2948.00 DKK valid until 2025-07-04
Use promo code "24108" to get your promotional price.
This item is not returnable. View return policy

Description

Periodate-aldehyde-activated horseradish peroxidase (HRP) enzyme and kit to label primary antibodies to obtain high-activity antibody-HRP conjugates.

Thermo Scientific™ EZ-Link™ Plus Activated Peroxidase Kit contains horseradish peroxidase enzyme that has been aldehyde-activated with periodate to enable high-efficiency labeling and preparation of protein or antibody conjugates.

Other amine-reactive chemistries, like glutaraldehyde, have a tendency to polymerize and produce lower amounts of viable conjugate. Sugar residues present on HRP have been oxidized with periodate to produce aldehydes that react with primary amines. Plus Activated Peroxidase is mixed with the protein to be coupled at a pH compatible with your protein or antibody. After incubating the reaction for one to two hours, the conjugate linkage is reduced and the activated HRP quenched with ethanolamine. The conjugate is purified by desalting or by dialysis. If you are preparing an antibody-HRP conjugate, use the Thermo Scientific Pierce Conjugate Purification Kit (Part No. 44920) to separate unreacted enzyme from the conjugate.

Highlights:

  • Reacts with readily accessible primary amines to form a covalent secondary amine bond
  • Can be stored for at least 12 months at -20°C
  • Consistent conjugation yields reliable conjugates
  • Enzyme activity is 120-200 units/mg
TRUSTED_SUSTAINABILITY

Specifications

Chemical Reactivity Amine
Quantity 5 x 1mg
Reactive Moiety Aldehyde
Solubility Water
Content And Storage Note: EZ-Link Plus Activated Peroxidase contains 1-3 moles of aldehyde per mole of peroxidase.

Storage: Upon receipt store product protected from moisture at -20°C. Product is shipped with an ice pack.
Form Solid
Product Line EZ-Link™
Label Type Enzyme Labeled
Label or Dye HRP (Horseradish Peroxidase)

Certificates

Certificates

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Lot Number Certificate Type Date Product Code
Lot NumberSPECS Certificate TypeCertificate of Analysis Date11/01/2023 Product Code31488

For Research Use Only. Not for use in diagnostic procedures.

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