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Thermo Scientific™ Pierce™ Protein G UltraLink™ Resin

Extremely durable polyacrylamide support with immobilized, recombinant Protein G for various IgG purification methods.

Brand: Thermo Scientific™ 53125

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Product Code. 10568923

3535.00 DKK / 2mL

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Description

Thermo Scientific™ Pierce Protein G UltraLink Resin is an extremely durable beaded polyacrylamide support to which recombinant Protein G has been immobilized for use in IgG purification methods.

The Protein G UltraLink Resin is used to bind and purify IgG in gravity-flow, spin-column or medium-pressure chromatography methods. Protein G is a popular antibody-binding protein that is especially useful for affinity purification of IgG from mouse, human and rat serum.

UltraLink Resin is an azlactone-activated polyacrylamide support that is hydrophilic, charge-free, high capacity, highly crosslinked, rigid and porous. This support is especially useful for medium pressure techniques when using large sample volumes requiring fast-flow techniques (FPLC) and large-scale applications. Unlike typical beaded agarose supports, UltraLink Support does not crush or collapse when subjected to high flow rates that result in pressures exceeding 25psi.

Highlights:

Immobilized Protein G – specifically binds IgG from several mammalian species, especially all isotypes of IgG from human, mouse and rat
Standard capacity – loading of immobilized Protein G provides a binding capacity of approx. 20mg human IgG per milliliter of resin
UltraLink Resin – acrylamide-based support provides an alternative to crosslinked beaded agarose supports for medium pressure applications

Properties of Thermo Scientific UltraLink Resin:

Support pH Stability: 1 to 13
Average Particle Size: 50 to 80 microns
Exclusion Limit: greater than 2,000,000 daltons
Average Surface Area: greater than 250m² per gram of beads
Average Pore Volume: greater than 1.2mL per gram of beads (> 60% of bead volume)
Pore Size: 500 to 1000 angstroms (50 to 100nm)
Maximum Linear Velocity: 3000cm per hour
Maximum Pressure: 100psi (6.9 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather than the pressure drop across the column.)

Specifications

Product Type	Resin
Column Type	UltraLink Affinity Resin
Stationary Phase	Protein G
Content And Storage	Upon receipt store product at 4°C. Products are shipped at ambient temperature.

Product Line	Pierce™, UltraLink™
Format	Bottle
Purification Target	IgG Antibodies
Quantity	2 mL

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Documents

Product Information

Manuals

User Guide: Protein G UltraLink Resin Version: B File size: 377.8 kB

Certificates

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot NumberZF383017	Certificate TypeCertificate of Analysis	Date22/06/2024	Catalog Number53125
Lot NumberYB361879	Certificate TypeCertificate of Analysis	Date28/02/2023	Catalog Number53125
Lot NumberXB333206	Certificate TypeCertificate of Analysis	Date22/03/2022	Catalog Number53125
Lot NumberWD313723	Certificate TypeCertificate of Analysis	Date26/04/2021	Catalog Number53125
Lot NumberVC302198	Certificate TypeCertificate of Analysis	Date14/03/2020	Catalog Number53125

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