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Invitrogen™ PureLink™ Microbiome DNA Purification Kit

Brand: Invitrogen™ A29790

2422.50 DKK valid until 2025-06-30
Use promo code "24108" to get your promotional price.

Product Code. 15449733

2850.00 DKK / 1 set

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Description

The Invitrogen™ PureLink™ Microbiome DNA Purification Kit uses proven PureLink™ spin column technology for robust yields of purified DNA ready for downstream PCR, sequencing or other applications. The highly efficient triple lysis approach, fast removal of inhibitors, and versatility makes PureLink the ultimate kit for microbiome research projects as well as programs aimed at rapid detection of pathogenic bacteria in various samples.

Efficient lysis of all microorganisms (including durable species with thicker and more complex cell walls) by a combination of heat, chemical, and mechanical disruption with specialized beads
Elimination of inhibitory compounds by precipitation using a novel cleanup buffer
Streamlined protocols for numerous biological samples
Recovery of highly pure DNA compatible with common downstream applications such as qPCR and next-generation sequencing

Certifications

Certificates of Analysis for each lot

Services

Product performance guaranteed for 1 year from date of sale

Specifications

Column Type	Spin Column
Isolation Technology	Silica Spin Column
Sample Type	Microbial Culture, Saliva, Soil, Stool, Host DNA from Stool, Swabs (Buccal, Vaginal, Skin, Rectal, or Environmental), Cells, Food and Environmental
Final Product Type	Microbial and host DNA
High-throughput Compatibility	Not High-throughput Compatible (Manual)
Shipping Condition	Room Temperature
Yield	≤25 μg

Content And Storage	• 40 mL S1 Lysis Buffer; room temperature • 5 mL S2 Lysis Enhancer; room temperature • 12.5 mL S3 Cleanup Buffer; room temperature • 45 mL S4 Binding Buffer; room temperature • 13 mL S5 Wash Buffer (concentrated); room temperature • 5 mL S6 Elution Buffer; room temperature • 50 PureLink Columns with Collection Tubes; room temperature • 100 PureLink Collection Tubes; room temperature • 50 Bead Tubes; room temperature
Scale	Mini
Elution Volume	50 to 200 μL
For Use With (Application)	PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
Quantity	50 Preps kit
Test Time	Up to 55 min. (Including sample homogenization)
Starting Material Amount	Buccal/Vaginal/Skin/Rectal/Environmental Swab: 1 swab Microbial Culture: ≤5 mL Saliva: ≤2 mL Soil: ≤2 g Stool: ≤2 g Urine: ≤10 mL

Product Suggestions Safety and Handling

Safety and Handling

Product Identifier

PureLink Microbiome DNA Purification Kit

Signal Word

Warning

Hazard Category

Acute toxicity Category 4
Serious eye damage/eye irritation Category 2
Skin corrosion/irritation Category 2

Hazard Statement

H302-Harmful if swallowed.
H315-Causes skin irritation.
H319-Causes serious eye irritation.
H332-Harmful if inhaled.

Precautionary Statement

P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
P264-Wash hands thoroughly after handling.
P270-Do not eat, drink or smoke when using this product.
P271-Use only outdoors or in a well-ventilated area.
P280-Wear protective gloves/protective clothing/eye protection/face protection.
P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
P302+P352-IF ON SKIN: Wash with plenty of water/soap.
P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P312-Call a POISON CENTER/doctor/if you feel unwell.
P330-Rinse mouth.
P332+P313-If skin irritation occurs: Get medical advice/attention.
P337+P313-If eye irritation persists: Get medical advice/attention.
P362+P364-Take off contaminated clothing and wash it before reuse.
P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

MIXTURE LIST-Contains : Guanidine hydrochloride, Thiocyanic acid

Documents

Product Information

Manuals

User Guide: PureLink Microbiome DNA Purification Kit (Soil Samples) Version: MAN0014331 Rev.A (10Sep2015) File size: 669.1 kB

User Guide: PureLink Microbiome DNA Purification Kit (Host DNA from Stool Samples) Version: MAN0014334 Rev.A (10Sep2015) File size: 3.5 MB

User Guide: PureLink Microbiome DNA Purification Kit (Rectal and Environmental Swab Samples) Version: MAN0014333 Rev.A (10Sep2015) File size: 703 kB

User Guide: PureLink Microbiome DNA Purification Kit (Transport Media and Microbial Culture Samples) Version: MAN0014332 Rev.A (10Sep2015) File size: 727.4 kB

User Guide: PureLink Microbiome DNA Purification Kit (Buccal, Vaginal, and Skin Swab Samples) Version: MAN0014268 Rev.A (10Sep2015) File size: 638.4 kB

User Guide: PureLink Microbiome DNA Purification Kit (Saliva and Urine Samples) Version: MAN0014267 Rev.A (10Sep2015) File size: 622.6 kB

Scientific Resources

Application Notes

Application Note: Rapid 2-hour workflow for detection of select bacteria in stool samples File size: 342.9 kB

Flyers

Flyer: PCR enzymes and sample prep kits for microbiome research File size: 388.3 kB

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