Invitrogen™ Rat TRPV1 (aa 1-21) Synthetic Peptide

Description

Rat VR1 (residues 1-21) Synthetic Peptide, PEP-094, from Invitrogen.

TRPV1 (Transient Receptor Potential Cation Channel Subfamily V Member 1) is a receptor for capsaicin, the main pungent ingredient in hot chili peppers, and elicits a sensation of burning pain by selectively activating sensory neurons. TRPV1 is a non-selective cation channel that is structurally related to members of the TRP family of ion channels such as TRPC3 and TRPC6. TRPV1 is permeable to calcium, magnesium, and monovalent cations of sodium and potassium. Structurally, TRPV1 contains 6 trans-membrane domains, a P-loop membrane domain and 3 ankyrin repeat domains. TRPV1 is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. TRPV1 seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. The expression of TRPV1 appears to be limited to neurons within the sensory ganglia. Four transcript variants encoding TRPV1 with variations in th 5′ UTR sequence have been described. Diseases associated with TRPV1 include Irritable Bowel Syndrome (IBS) and Bladder Disease. PEP-094 is a 21 amino acid synthetic peptide whose sequence corresponds to residues 1-21 of rat VR1 conjugated to KLH. The sequence of this peptide is (amino to carboxy terminus): M (1)-E-Q-R-A-S-L-D-S-E-E-S-E-S-P-P-Q-E-N-S-C (21)This peptide may be used for neutralization and control experiments with the polyclonal antibody that reacts with this product and endogenous VR-1 catalog &Num; PA1-747. Using a solution of peptide of equal volume and concentration to the corresponding antibody will yield a large molar excess of peptide (∽70-fold) for competitive inhibition of antibody-protein binding reactions.Reconstitute with 0.1 mL of distilled water.