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Ambion™ RiboMinus™ Eukaryote Kit for RNA-Seq

The RiboMinus™ Eukaryote Kit for RNA-Seq provides truly complete transcriptome isolation for next generation sequencing analysis.

Brand: Ambion™ A1083708

9478.12 DKK valid until 2025-12-31
Use promo code "24340" to get your promotional price.

Product Code. 10063854

11570.00 DKK / Each

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This item is not returnable. View return policy

Description

The RiboMinus™ Eukaryote Kit for RNA-Seq provides truly complete transcriptome isolation for next generation sequencing analysis by offering a method for selective depletion of up to 99% of the 5S, 5.8S, 18S and 28S ribosomal RNA from total RNA versus poly-A or cap binding methods, which can introduce bias and select for only part of the transcriptome.

The RiboMinus™ Eukaryote Kit for RNA-Seq Offers:
• A simple, yet selective depletion procedure that uses a sequence-specific probe to capture rRNA
• Rapid and efficient removal of rRNA-probe complex using magnetic bead technology

Three Steps to an rRNA-free Preparation Suitable for Transcriptome Analysis
The removal of large ribosomal RNA using the RiboMinus™ kit is a reliable and robust three-step procedure. Hybridize the RNA sample to the RiboMinus™ Probe in a water bath or heat block, then remove probe-rRNA complexes using the RiboMinus™ magnetic beads included in the kit. Concentrate the depleted RNA preparation by ethanol precipitation, or by using the RiboMinus™ Concentration Module (Cat. No. K155005).

Specifications

Content And Storage	Each kit contains: • 6 mL RiboMinus™ Magnetic Beads • 80 μL RiboMinus™ Eukaryote Probe (15 pmol/μL) • 10 mL Hybridization Buffer • 12 mL DEPC-treated (RNase-free) water Store at 4°C.
Sample Type	Total RNA
High-throughput Compatibility	High-throughput Compatible
No. of Reactions	8 Preps
Quantity	8 Preps (10 μg⁄prep)
Shipping Condition	Wet Ice
Target	Transcriptome RNA
Yield	1 μg

Isolation Technology	Magnetic Bead
Final Product Type	Transcriptome RNA
For Use With (Application)	Microarray Analysis, Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Sequencing, Northern Blotting, cDNA Library Construction
Product Line	RiboMinus™
Sequencing Type	Transcriptome Sequencing
Starting Material Amount	2-10 μg Total RNA
System Type	RiboMinus

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Ambion™ RiboMinus™ Eukaryote Kit for RNA-Seq

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