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Thermo Scientific™ RPMI 1640 Medium for SILAC

Analyze protein expression by mass spectrometry using these media or additives for stable isotope labeling with amino acids in cell culture (SILAC).

Brand:  Thermo Scientific™ 88365

384.01 DKK valid until 2025-03-29
Use promo code "24111" to get your promotional price.

Product Code. 15626868

  • 576.00 DKK / 500mL

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Description

Description

Thermo Scientific RPMI Media for SILAC is optimized for use with stable isotope labeling with amino acids in cell culture (SILAC) to analyze protein expression by mass spectrometry (MS).

Stable isotope labeling with amino acids in cell culture (SILAC) is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of “heavy” 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.

This SILAC media is used together with heavy and light amino acids for the differential isotopic labeling of cells for SILAC analysis. SILAC media supplemented with heavy amino acids are capable of nearly 100% label incorporation into proteins of living cells.

Features of RPMI Media for SILAC:
  •  Flexible—liquid media deficient in both L-lysine and L-arginine, allowing for more complete proteome coverage through multiple isotopic amino acid labeling
  • High quality—media are sterile, endotoxin-free and cell culture-compatible
TRUSTED_SUSTAINABILITY
Specifications

Specifications

Mass Spectrometer
Media
Mass Isotope of Amino Acid
Protein Samples
Mass Spectrometry
500 mL
Protein samples
1 x 500 mL, store at 2–8°C
Room Temperature
Liquid Medium
Cell Culture
Liquid
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5 results found
Lot Number Certificate Type Date Catalog Number
Lot NumberZH4372311 Certificate TypeCertificate of Analysis Date07/09/2024 Catalog Number88365
Lot NumberZB4117921 Certificate TypeCertificate of Analysis Date09/03/2024 Catalog Number88365
Lot NumberYJ3982311 Certificate TypeCertificate of Analysis Date11/11/2023 Catalog Number88365
Lot NumberXD3561891 Certificate TypeCertificate of Analysis Date10/03/2023 Catalog Number88365
Lot NumberXG3639981 Certificate TypeCertificate of Analysis Date28/02/2023 Catalog Number88365
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Product Title
Thermo Scientific™ RPMI 1640 Medium for SILAC > Starting material: Protein samples; Quantity: 500mL

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