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Gibco™ RPMI 1640 Medium, low HEPES, low bicarbonate, no glutamine
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Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer.

Brand:  Gibco™ 42402016

Product Code. 11574496

  • 228.00 DKK / 500mL

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Descrizione

Descrizione

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI 1640 is modified as follows:

  • With: Phenol Red, HEPES
  • Without: L-glutamine

The complete formulation is available.

Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12, and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.

Product Use

RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

TRUSTED_SUSTAINABILITY
Specifica

Specifica

ISO 13485 standards, FDA
CO2
Animal Origin-free
Mammalian Cell Culture
RPMI 1640
Sterile-filtered
No Glutamine, No Sodium Pyruvate
cGMP-compliant under the ISO 13485 standard
7 to 7.4
500 mL
Storage: 2°C to 8° C, protect from light
Shipping: Ambient
Shelf life: 12 months from date of manufacture
Roswell Park Memorial Institute (RPMI) 1640 Medium, 500mL, Liquid, 1 X Concentration, 7.0 to 7.4 pH, With Phenol Red, HEPES, 12 Months Shelf Life, 270 to 300mOsm/kg Osmolality, For Mammalian Cell Culture
1X
Liquid
Standard Serum Supplementation
HEPES, Phenol Red, Sodium Bicarbonate
Mammalian cell culture
270–300 mOsm/kg
Gibco™
Room Temperature
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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.