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Thermo Scientific™ SalI (10 U/μL)

The SalI restriction enzyme recognizes G^TCGAC sites and cuts best at 37°C in O buffer.

Brand: Thermo Scientific™ ER0642

1450.07 DKK valid until 2025-03-29
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Product Code. 10242440

2175.00 DKK / Pack of 5

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Description

Lambda DNA digested with SalI, 0.7% agarose, 2 cleavage sites

conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5'...G▵T C G A C...3'

3'...C A G C T▵G...5'

Conditions for 100% Activity

1X Buffer O:50mM Tris-HCl (pH7.5 at 37°C), 10mM MgCl₂, 100mM NaCl and 0.1mg/mL BSA
Incubate at 37°C

Storage Buffer

SalI is supplied in:10mM Tris-HCl (pH7.4 at 25°C),100mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage

After 50-fold overdigestion with SalI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects

Dam: never overlaps — no effect
Dcm: never overlaps — no effect
CpG: completely overlaps — blocked
EcoKI: never overlaps — no effect
EcoBI: never overlaps — no effect

Digestion of Agarose-embedded DNA

Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours

Compatible Ends

Eco88I, SmoI, XhoI

Note:

Low salt, high glycerol (>5%) concentrations, pH >8.0 or a large excess of enzyme may result in star activity. Supercoiled forms of pBR322 and pUC require 10-fold overdigestion with SalI to achieve complete digestion. Incubation at 25°C results in 50-75% activity.

Specifications

Concentration	10 U/μL
Enzyme	Sal I
Sensitive to Heat Inactivation	Yes
Type IIS RE	No
Product Type	Restriction Enzyme

Methylation Sensitivity	CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Compatible Buffer	10X Buffer O
Optimal Reaction Temperature	37°C
Quantity	5 x 1,500 units
Research Category	Traditional Cloning

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Documents

Product Information

Manuals

User Guide: SalI, 10 U/uL, 5x1500U Version: Jan. 2015 File size: 64.7 kB

User Guide: SalI, 10 U/uL, 2000U Version: Jan. 2015 File size: 64.7 kB

Certificates

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number2797790	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberER0642
Lot Number2821629	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberER0642
Lot Number2836259	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberER0642
Lot Number2840957	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberER0642
Lot Number2842059	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberER0642

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Thermo Scientific™ SalI (10 U/μL)

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Thermo Scientific™ SalI (10 U/μL)

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