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Thermo Scientific™ Tango™ HTR1A-bla U2OS Cells

Tango™ HTR1A-bla U2OS cells contain the human Serotonin Type 1a receptor (HTR1A) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.

Brand:  Thermo Scientific™ K1857

Product Code. 10175694

  • 91200.00 DKK / Each
Estimated Shipment: 15-01-2025
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Description

Description

Tango™ HTR1A-bla U2OS cells contain the human Serotonin Type 1a receptor (HTR1A) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase reporter gene under the control of a UAS response element. Tango™ HTR1A-bla U2OS DA cells have been functionally validated for Z' factor and EC50 concentrations of 5-CT (Figure 1). In addition, Tango™ HTR1A-bla U2OS cells have been tested for assay performance under variable conditions.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

NM_000524
Dividing Cells
Tail from AVPR2 GPCR
FRET
G-Protein Coupled Receptor (GPCR)
Single Cell Clone Origin
8 x 106 Cells
5HT1A, HTR1A
U2OS
GPCR
Cell-based beta-arrestin recruitment
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.

Includes:
• Tango™ HTR1A-UAS-bla CHO-K1 cells
• Tango™ HTR1A-UAS-bla CHO-K1 Assay Protocol
• Certificate of Analysis

The Tango™ HTR1A-UAS-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Fluorescence
HTR1A
HTR1A-UAS-bla CHO-K1 cells, HTR1A-UAS-bla CHO-K1 Assay Protocol, Certificate of Analysis
Tango™
End Point
Dividing Cells
Tango™
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