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Thermo Scientific™ pMCS minP-Tluc16 Hygro Vector for Luciferase Assays
Insert promoter sequences for genes of interest into Thermo Scientific™ Tluc16 Vectors to measure gene regulation using the intracellular TurboLuc™ 16 (Tluc16) luciferase reporter in luciferase assays.
Brand: Thermo Scientific™ 88256
This item is not returnable.
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Description
The Thermo Scientific pMCS Tluc16 Vectors are multiple cloning site (MCS) vectors designed to accept a promoter sequence for study of gene regulation using the intracellular TurboLuc™16 (Tluc16) luciferase reporter.
- Intracellular Tluc16 luciferase gene, optimized for high expression in mammalian systems
- Multiple cloning sites provide versatility for transfer of regulatory elements from one vector to another
- Synthetic polyA terminator and Transcriptional Pause Site (TPS) included upstream of MCS to minimize non-specific transcriptional read-through
- Dual-destabilization (DD) technology reduces accumulation of Tluc16 luciferase mRNA and protein in cells, enhancing the responsiveness of the assay
- Optimized minimal core promoter (minP) and 5' UTR region for efficient expression of Tluc16 luciferase
- Hygromycin resistance gene for use as selection marker to generate mammalian stable cell lines
- Prepared pNF-κB, and pCRE Tluc16-DD Vectors are designed to monitor the activation of NFκB protein and cAMP-binding protein (CREB) in mammalian cells
- Prepared pEF1α variety includes a modified version of the Elongated Factor 1α (EF1α) promoter for constitutive Tluc16 luciferase expression in mammalian cells
Specifications
Hygro Vector | |
Transfection | |
Store in freezer (-5 to -30°C). | |
Hygromycin | |
minP |
Restriction Enzyme ⁄ MCS | |
10 μg | |
TurboLuc™16 Luciferase | |
Reporter Assays |
For Research Use Only. Not for use in diagnostic procedures.