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BD Biosciences™ Transcription Factor Phospho Buffer Set (RUO)
Designed to enable robust and simultaneous flow cytometric analysis of various cell surface antigens, transcription factors, and phosphorylated signal proteins in cells derived from both human and mouse tissues.
Brand: BD Biosciences™ 563239
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Description
- Designed to enable robust and simultaneous flow cytometric analysis of various cell surface antigens, transcription factors, and phosphorylated signal proteins in cells derived from both human and mouse tissues.
- This includes fresh, fixed, and frozen human peripheral blood mononuclear cells.
- The BD Pharmingen™ TFP Buffer Set has been successfully applied to the multiparameter flow cytometric analysis of phosphorylated Stat signaling responses to cytokines made by human and mouse T regulatory cell and conventional T cell subsets, and other leucocyte types defined by certain cell surface markers, transcription factors (FoxP3 and T-bet), and physical characteristics.
- While not all antibody compatibilities are known, cell signaling responses to different stimuli should also be amenable to analysis using this buffer set and antibodies specific for other cell surface markers, transcription factors, and types of downstream phosphorylated signaling proteins.
- This buffer system is backwards compatible with many BD reagents, including antibodies conjugated with new fluorescent dyes (eg, BD Horizon™ BV421, BV510, PE-CF594, V450, and V500).
Specifications
| Transcription Factor Phospho Buffer Set | |
| 1 Set, 100 Tests, 75 mL 0.75X TFP Diluent Buffer, 25 mL 4X TFP Fix/Perm Buffer, 125 mL Perm Buffer III, 150 mL 5X TFP Perm/Wash Buffer | |
| 75 mL 0.75X TFP Diluent Buffer, 25 mL 4X TFP Fix/Perm Buffer, 125 mL Perm Buffer III, 150 mL 5X TFP Perm/Wash Buffer |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Transcription Factor Phospho Buffer Set
Signal Word
- Danger
Hazard Category
- Acute toxicity Category 3
- Acute toxicity Category 4
- Carcinogenicity Category 1A
- Serious eye damage/eye irritation Category 1
- Serious eye damage/eye irritation Category 2
- Flammable liquid Category 2
- Germ cell mutagenicity Category 2
- Skin sensitiser Category 1
- Specific target organ toxicity after single exposure Category 1
- Specific target organ toxicity Category 2
Hazard Statement
- H225-Highly flammable liquid and vapour.
- H301-Toxic if swallowed.
- H302-Harmful if swallowed.
- H311-Toxic in contact with skin.
- H312-Harmful in contact with skin.
- H315-Causes skin irritation.
- H317-May cause an allergic skin reaction.
- H318-Causes serious eye damage.
- H331-Toxic if inhaled.
- H335-May cause respiratory irritation.
- H341-Suspected of causing genetic defects.
- H350-May cause cancer.
- H370-Causes damage to organs.
- H371-May cause damage to organs.
Precautionary Statement
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Supplemental information
- MIXTURE LIST-Contains : methanol, formaldehyde