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Cytiva NAP™ Columns, NAP-10
Use for rapid and efficient purification of DNA and oligonucleotides (>10-mer) in less than 15 minutes by gravity flow. Cytiva Life Sciences™ illustra™ NAP™ Columns are disposable columns prepacked with Sephadex™ G-25 DNA Grade
Includes | Complete step-by-step protocol identifying the fixed volumes needed for equilibration, washing and sample elution |
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Product Type | NAP-10 |
For Use With (Application) | Rapid and efficient purification of DNA and Oligonucleotides |
Cytiva NAP™ Columns, NAP-25
Use for rapid and efficient purification of DNA and oligonucleotides (>10-mer) in less than 15 minutes by gravity flow. Cytiva Life Sciences™ illustra™ NAP™ Columns are disposable columns prepacked with Sephadex™ G-25 DNA Grade
Includes | Complete step-by-step protocol identifying the fixed volumes needed for equilibration, washing and sample elution |
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Product Type | NAP-25 |
For Use With (Application) | Rapid and efficient purification of DNA and Oligonucleotides |
Cytiva PuReTaq Ready-To-Go™ PCR Beads
Pre-mixed, pre-dispensed reaction beads optimized for performing standard PCR amplifications. Cytiva Lifescience™ illustra™ PuReTaq Ready-To-Go™ PCR Beads are stable at ambient temperature.
Quantity | 1 Kit |
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For Use With (Application) | Standard PCR |
Cytiva ExoProStar™ 1-step
Use for enzymatic PCR clean-up. Cytiva Lifescience™ Illustra ExoProStar™ 1-step with illustra Exonuclease I and Alkaline Phosphatase combined in one tube to remove unincorporated primers and dNTPs.
Description | Enzymatic PCR clean up technology with illustra Exonuclease I and Alkaline Phosphatase combined in one tube to remove unincorporated primers and dNTPs. For maximum convenience comes in a single tube. |
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Product Type | illustra ExoProStar 1-Step |
Isolation Technology | Enzymatic PCR Clean-up Technology |
Quantity | 1 Each |
Yield | 100% recovery of PCR product |
For Use With (Application) | Remove unincorporated primers and dNTPs |
Cytiva NAP™ Columns, NAP-5
Use for rapid and efficient purification of DNA and oligonucleotides (>10-mer) in less than 15 minutes by gravity flow. Cytiva Life Sciences™ illustra™ NAP™ Columns are disposable columns prepacked with Sephadex™ G-25 DNA Grade
Includes | Complete step-by-step protocol identifying the fixed volumes needed for equilibration, washing and sample elution |
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Product Type | NAP-5 |
For Use With (Application) | Rapid and efficient purification of DNA and Oligonucleotides |
Cytiva MicroSpin™ G-25 Columns
Use for the rapid purification of DNA >10 base pairs in length in a wide range of applications. Cytiva Life Sciences™ llustra™ MicroSpin™ G-25 Columns are ideal for desalting, buffer exchange, and removal of unincorporated nucleotides from end-labeled oligonucleotides.
Cytiva MicroSpin™ S-400 HR Columns
Use for rapid purification of PCR products (>200 bp) from unincorporated primers (<32-mers) and nucleotides. Cytiva Life Sciences™ illustra™ MicroSpin™ S-400 HR Columns use spin-column chromatography for quick results.
Cytiva Amersham™ ECL™ Blocking Agent
Use for blocking nitrocellulose and PVDF membranes. Cytiva Life Sciences™ Amersham™ ECL™ Blocking Agent blocks membranes to be used with ECL and ECL Plus™ Western blotting detection reagents.
Cytiva SeraSil-Mag Silica Coated Superparamagnetic Beads
SeraSil-Mag beads for nucleic acid isolation deliver high purity DNA extraction in NGS applications.
Content And Storage | Room Temperature |
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Description | Storage Solution: Water + 0.05% sodium azide |
Product Type | Silica coated, core-shell structure |
Shelf Life | 24 months at room temperature |
Concentration | 20 mg/mL in water (0.05% sodium azide) |
For Use With (Application) | Chaotropic salt chemistry and vortex mixing, roller mixing and sonication |
Cytiva ProbeQuant™ G-50 Micro Columns
For rapid purification of labeled DNA (>20 bases) from unincorporated labeled nucleotides in a volume of 25 to 50μL. Cytiva Life Sciences™ ProbeQuant™ G-50 Micro Columns use spin-column chromatography.
Cytiva TempliPhi™ 100/500 Amplification Kit
Use amplified DNA directly for cycle sequencing without purification. Cytiva illustra TempliPhi DNA Amplification Kits efficiently prepare DNA sequencing templates from circular DNA.
Includes | 0.5mL Sample Buffer, 0.5mL Reaction Buffer, 20μL Enzyme mix and 50μL positive control pUC19 DNA |
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Content And Storage | The product can be stored for 2 months or less at -20°C. For storage longer than 2 months, store at -70°CThaw components on ice and maintain at 0–4°C during handling. DO NOT warm above 4°C prior to amplification |
Description | illustra TempliPhi DNA Amplification Kits efficiently prepare DNA sequencing templates from circular DNA. |
For Use With (Application) | Sequencing Template Amplification Kits and Reagents |
Test Time | 4-18 h depending on starting material |
Cytiva GenomiPhi V2 DNA Amplification Kit
Amplify whole genomes with high efficiency and representation. Cytiva Life Sciences™ illustra™ GenomiPhi V2 DNA Amplification Kit provides whole genome amplification with yields of 4 to 7μg.
Content And Storage | Store the kit at -70°C.The enzyme mix must be stored at -70°C; all other components may be stored at -20°C. Thaw components on ice and maintain at 0°C to 4°C during handling. |
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Description | Illustra GenomiPhi V2 DNA Amplification Kit offers highly efficient and representative whole-genome amplification with 4 to 7 μg yield from nanogram amounts of DNA sample. |
Product Type | illustra GenomiPhi V2 DNA Amplification Kit |
Size | > 10 kb |
For Use With (Application) | Genomic DNA Amplification |
Test Time | 1.5-2 h |
Cytiva Ready-To-Go™ GenomiPhi V3 DNA Amplification Kit
Highly efficient and representative whole-genome amplification with 40 to 60μg yield from nanogram amounts of DNA sample. Cytiva Lifescience™ illustra™ Ready-To-Go™ GenomiPhi V3 DNA Amplification Kit is part of the Phi29 DNA polymerase family of products.
Cytiva MicroSpin™ S-200 HR Columns
For rapid purification of labeled single-stranded or double-stranded DNA fragments ≥50 bases in length in a volume of 25 to 100μL using spin-column chromatography
Cytiva Taq DNA Polymerase (cloned)
For PCR and other procedures requiring DNA polymerase activity at elevated temperatures
Content And Storage | 100mM Tris HCl, pH 9, 15mM MgCl2 and 500mM KCl. Store at -20°C |
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Purity or Quality Grade | Free from detectable nonspecific nuclease and Taq I restriction endonuclease activities. |
pH | 7.5 |
Concentration | 5000U/mL |
For Use With (Application) | Standard PCR |