Life Science Research ELISA Kits

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Assay Thiol levels in protein and peptide containing biological buffers including Tris, phosphate, and citrate buffers.

Shipping Condition	Approved for shipment on Wet or Dry Ice
Content And Storage	96-well plate(s), standard, other assay specific reagents, buffers, and lot-specific technical data sheet. Store at –20°C.
For Use With (Equipment)	Microplate Reader
Gene ID (Entrez)	Non-Gene
Gene Symbol	Non-Gene
Target Kit Named	Thiol

a 4.5 hour solid phase ELISA designed to measure Cystatin B levels in cell culture supernates, serum, plasma, saliva, urine, and human milk.

Rabbit Monoclonal Antibody Pair

Host Species	Rabbit
Target Species	Human
Conjugate	HRP
Gene Symbol	CST2
Assay Sensitivity	4.69-300 pg/mL
Storage Requirements	Storage is content dependent.
Assay Range	4.7 pg/mL
For Use With (Application)	Sandwich ELISA
Assay	Sandwich ELISA
Regulatory Status	RUO
Target	Cystatin SA
Specificity	This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human Cystatin SA.
Kit Contents	Detection Antibody: 0.25 mg/mL rabbit anti-Cystatin SA monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % glycerol, pH 7.4). Capture Antibody: 1.0 mg/mL of rabbit anti-Cystatin SA monoclonal antibody (in PBS, pH 7.4). Standard: Each vial contains 15 ng of recombinant Cystatin SA.
Gene Alias	cystatin 2, cystatin S5, cystatin SA, Cystatin-2, Cystatin-S5, cystatin-SA, cysteine-proteinase inhibitor, MGC71924, salivary cysteine (thiol) protease inhibitor
Product Type	Antibody Pairs
Gene ID (Entrez)	1470

Mouse Monoclonal Antibody Pair

Immunoassay Kit Format	Sandwich ELISA Kit
Host Species	Mouse
Target Species	Human
Conjugate	HRP
Gene Symbol	CST2
Assay Sensitivity	2.34-150 pg/mL
Storage Requirements	Storage is content dependent.
Assay Range	2.4 pg/mL
For Use With (Application)	Sandwich ELISA
Assay	Sandwich ELISA
Regulatory Status	RUO
Target	Cystatin SA
Specificity	This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human Cystatin SA.
Kit Contents	Standard: Each vial contains 37 ng of recombinant Cystatin SA. Capture Antibody: 0.5 mg/mL of mouse anti-Cystatin SA monoclonal antibody, Detection Antibody: 0.5 mg/mL mouse anti-Cystatin SA monoclonal antibody conjugated to horseradish-peroxidase (HRP).
Gene Alias	cystatin 2, cystatin S5, cystatin SA, Cystatin-2, Cystatin-S5, cystatin-SA, cysteine-proteinase inhibitor, MGC71924, salivary cysteine (thiol) protease inhibitor
Product Type	Antibody Pairs
Gene ID (Entrez)	1470
Technique	1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in CBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 150 pg/mL is recommended.

ELISA

Conjugate	HRP
Interassay CV	<12%
Intraassay CV	<10
Gene Symbol	KNG1
Total Assay Time	4 hrs 45 mins
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	24 ng/mL
Storage Requirements	2°C to 8°C
Assay Range	24 to 6,000 ng/mL
Test Time	1 hrs 20 mins
Accession Number	Alpha-1-MAP; alpha-2-thiol proteinase inhibitor; BDK; BK; Bradykinin; fitzgerald factor; high molecular weight kininogen; H-kininigen; HMWK; Ile-Ser-Bradykinin; Kallidin I; Kallidin II; kininogen; kininogen 1; kininogen 1-like 1; kininogen-1; Kininogen-1 heavy chain; Kininogen-1 light chain; Kng; KNG1; Kng1l1; L-kininogen; low molecular weight (LMW) T-kininogen II; Low molecular weight growth-promoting factor; Lysyl-bradykinin; Major acute phase protein; major acute phase protein (alpha-1-MAP); Thiostatin; T-kinin; T-kininogen 2; T-kininogen 2 heavy chain; T-kininogen 2 light chain; T-kininogen II; T-kininogen II heavy chain; T-kininogen II light chain; Williams-Fitzgerald-Flaujeac factor
Regulatory Status	RUO
Target	Kininogen 1
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3827
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP