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Protein Purification

Protein Purification

Kits, columns, gels, reagents, enzymes, inhibitors, and other assorted products for use with protein purification procedures.
Protein Purification Reagents and Kits (1,543)
Magnetic Affinity Separation Particles (259)
Protease Inhibitors and Phosphatase Inhibitors (193)
Gel Filtration Media and Columns (112)
Spin Columns and Gravity-flow Columns (112)
Miscellaneous Immobilization Supports (110)
Cell Lysis Detergents (90)
In vitro Protein Expression Systems (43)
Biotin-Binding Affinity Resins and Beads (34)
Affinity Gels (33)
Miscellaneous Immobilized Affinity Ligands (26)
Cell Lysis Enzymes (19)

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1 – 15 of 1,636 results
1

Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

EDGE
Flow Rate 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Type Pre-packed Column
Content And Storage 4°C to 30°C
Description HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
2

Thermo Scientific™ Pierce™ Protease Inhibitor Mini Tablets, EDTA-free

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments

3

Thermo Scientific™ Lysozyme

Degrade unwanted bacterial cell wall in protein or nucleic acid extracts

4

Cytiva Gel Filtration Calibration Kits

Calibrate gel filtration columns simply, reliably. Cytiva Life Sciences™ Gel Filtration Calibration Kits contain a well-defined series of protein standards.

Promotions are available
Content And Storage 2° to 8°C
Product Type Calibration Kit
For Use With (Equipment) High-resolution media and prepacked column
Quantity Each
For Use With (Application) Calibration of high-resolution gel filtration media and column such as Superdex™, Superose and Sephacryl
5

Thermo Scientific™ NP-40 Surfact-Amps™ Detergent Solution

Thermo Scientific™ Surfact-Amps NP-40 Detergent Solution is highly-purified NP-40 detergent stabilized and supplied as a 10% solution.

EDGE
Form Liquid
Reagent Type Detergent Solution
Product Line Surfact-Amps™
6

Invitrogen™ Dynabeads™ Protein A for Immunoprecipitation

Dynabeads Protein A beads are uniform, 2.8-μm superparamagnetic beads with recombinant Protein A (∼45 kDa) covalently coupled to the surface.

EDGE
Shipping Condition Ambient temperature
Content And Storage Dynabeads Protein A are supplied in PBS, pH 7.4 w/0.01% Tween-20, 0.09% NaN3 Store at 2–8°C.
Purity or Quality Grade For Research Use Only
Description Recombinant Protein A covalently bound to Dynabeads
Surface Functionality Protein A
Material Polystyrene
Concentration 30 mg/mL
For Use With (Application) Immunoprecipitation
High-throughput Compatibility High-throughput Compatible
Ligand Type Protein A
Type Superparamagnetic Beads
Target Antibodies
Format Beads in suspension
For Use With (Equipment) KingFisher™ Sample Purification System, DynaMag™ magnets
Diameter (Metric) 2.8 μm
Certifications/Compliance ISO9001 and ISO13485
Uniformity Monosized 2.8 mm (CV <5%)
Product Line Dynabeads™
7

Invitrogen™ Dynabeads™ M-270 Epoxy

Dynabeads™ M-270 Epoxy beads are 2.8 μm superparamagnetic beads containing surface epoxy groups.

Promotions are available
EDGE
Shipping Condition Room Temperature
Type Epoxy Bead
Purity or Quality Grade Research Grade
Format Freeze Dried
Surface Functionality Epoxy
Sample Type Any Sample Type
Diameter (Metric) 2.8 μm
Shelf Life 24 months from date of manufacture
Elution Volume Min 5 μL
Product Line DYNAL™, Dynabeads™
8

Thermo Scientific™ Aprotinin Protease Inhibitor

Customize your protease inhibitor cocktail formulation with these small packages of individual protease inhibitor peptides and compounds.

EDGE
9

Cytiva PD MiniTrap™ G-25 Sample Preparation Columns

Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

EDGE
10

Invitrogen™ Dynabeads™ MyOne™ Carboxylic Acid

Surface carboxylic acid groups allow covalent amide bond formation to proteins/peptides via primary amino- or sulphydryl groups.

EDGE
Shipping Condition Room Temperature
Type Carboxylic Acid Magnetic Bead
Content And Storage Store in refrigerator (2–8°C).
Purity or Quality Grade Research Grade
Format Beads in solution
Surface Functionality Carboxylic Acid
Sample Type Any Sample Type
Diameter (Metric) 1 μm
For Use With (Application) Protein Purification and Isolation
High-throughput Compatibility High-throughput Compatible
Elution Volume Min 5 μL
Product Line Dynabeads™, MyOne™
11

Cytiva HisTrap™ FF Columns: 16 x 25mm

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

EDGE
Flow Rate <20 mL/min.
Type Pre-packed Column
Content And Storage 4°C to 30°C, 20% Ethanol
Diameter (English) Inner 0.6 in.
Description HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Diameter (Metric) Inner 16 mm
Material Polypropylene
Support Type 6% Cross-linked Agarose
12

Cytiva PreScission™ Protease

Use for site-specific separation of the GST tag from proteins expressed using pGEX-6P vectors. Cytiva Life Sciences™ PreScission™ Protease G allows simultaneous protease immobilization and cleavage of GST fusion proteins.

13

Cytiva HiTrap™ Capto™ Adhere Columns

Designed to remove contaminants in post-protein A purification

EDGE
Particle Size 75μm
Buffer Aqueous Buffer
Ligand Type N-benzyl-n-methyl Ethanolamine
Height (Metric) 25mm
Content And Storage 4° to 30°C
pH Range 3 to 12
Product Type HiTrap Capto adhere column
Column Type Affinity Column
Material Polypropylene
For Use With (Application) Easy screening and convenient process development
Support Type Highly Cross-linked Agarose
Height (English) 0.98 in.
14

Invitrogen™ DynaMag™-2 Magnet

The DynaMag-2 magnet combines a strong magnetic attraction for efficient separations in standard microcentrifuge tubes with a flexible and smart ergonomic design.

15

Invitrogen™ Dynabeads™ Protein G for Immunoprecipitation

Dynabeads Protein G beads are uniform, 2.8-μm superparamagnetic beads with recombinant Protein G (∼17 kDa) covalently coupled to the surface.

Shipping Condition Ambient temperature
Content And Storage Dynabeads Protein G are supplied in PBS, pH 7.4 w/0.01% Tween-20, 0.09% NaN3 Store at 2–8°C.
Purity or Quality Grade For Research Use Only
Description Recombinant Protein G covalently bound to Dynabeads
Surface Functionality Protein G
Material Polystyrene
Concentration 30 mg/mL
For Use With (Application) Immunoprecipitation
High-throughput Compatibility High-throughput Compatible
Ligand Type Protein G
Type Superparamagnetic Beads
Target Antibodies
Format Beads in suspension
For Use With (Equipment) KingFisher™ Sample Purification System, DynaMag™ magnets
Diameter (Metric) 2.8 μm
Certifications/Compliance ISO9001 and ISO13485
Uniformity Monosized 2.8 mm (CV <5%)
Product Line Dynabeads™