Protein Purification Reagents and Kits
Protein Purification Reagents and Kits
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Filtered Search Results
Cytiva HisTrap™ HP Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
Flow Rate | 4 mL/min. (1 mL), 5 mL/min. (5 mL) |
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Type | Pre-packed Column |
Content And Storage | 4°C to 30°C |
Description | HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery. |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Material | Polypropylene |
Support Type | 6% Highly Cross-linked Agarose |
Cytiva PD MiniTrap™ G-25 Sample Preparation Columns
Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.
Cytiva HisTrap™ FF Columns: 16 x 25mm
Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.
Flow Rate | <20 mL/min. |
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Type | Pre-packed Column |
Content And Storage | 4°C to 30°C, 20% Ethanol |
Diameter (English) Inner | 0.6 in. |
Description | HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Diameter (Metric) Inner | 16 mm |
Material | Polypropylene |
Support Type | 6% Cross-linked Agarose |
Cytiva PreScission™ Protease
Use for site-specific separation of the GST tag from proteins expressed using pGEX-6P vectors. Cytiva Life Sciences™ PreScission™ Protease G allows simultaneous protease immobilization and cleavage of GST fusion proteins.
Particle Size | 75μm |
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Buffer | Aqueous Buffer |
Ligand Type | N-benzyl-n-methyl Ethanolamine |
Height (Metric) | 25mm |
Content And Storage | 4° to 30°C |
pH Range | 3 to 12 |
Product Type | HiTrap Capto adhere column |
Column Type | Affinity Column |
Material | Polypropylene |
For Use With (Application) | Easy screening and convenient process development |
Support Type | Highly Cross-linked Agarose |
Height (English) | 0.98 in. |
Cytiva StrepTrap XT Chromatography Column
StrepTrap XP is a column packed with Strep-Tactin™ XT Sepharose resin to purify Strep-tag™ II and Twin-Strep-tag™ recombinant proteins.
Height (Metric) | 25 mm |
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Column Type | Affinity Column |
For Use With (Application) | Purification of recombinant proteins tagged with Strep-tag™ II and Twin-Strep-tag™ |
Cytiva HiTrap™ Capto™ AVB
Capto™ AVB is based on a rigid base matrix, delivering excellent pressure-flow properties to AAV production
Shipping Condition | Ambient |
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Height (Metric) | 2.5 cm |
Content And Storage | Store at 4°C to 8°C |
Column Type | Affinity Column |
dimMatrix | Capto AVB |
Cytiva™ MabSelect™ VL Protein L Resin
The MabSelect™ VL resin is a protein L resin for affinity capture of antibody Fabs, bispecifics, and other variants containing a kappa light chain.
Cytiva™ HiTrap™ Chromatography Columns with MabSelect™ VL Protein L Resin
Protein L affinity chromatography columns for capture of antibody Fabs, bispecifics, and other variants containing a kappa light chain.
Height (Metric) | 25 mm |
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Column Type | Affinity Column |
For Use With (Application) | Antibody Affinity Chromatography |
Max. Pressure | 5 bar |
Height (English) | 1 in. |
Cytiva Superose™ 6 Increase Columns
High-resolution size-exclusion chromatography for studying large proteins and complexes. Cytiva Lifescience™ Superose™ 6 Increase Columns delivers high quality in size exclusion chromatography (SEC).
Particle Size | 8.6μm |
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Ligand Type | Cross-linked agarose |
Content And Storage | 4° to 30° in 20% ethanol |
pH Range | 3 to 12 |
Column Type | Gel Filtration Column |
Material | Borosilicate glass |
Max. Pressure | 435 psi |
Cytiva HisTrap™ FF Crude Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cell lysates. Cytiva Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
Particle Size | 45 to 165 μm |
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Type | Pre-packed Column |
Content And Storage | 4°C to 30°C |
pH Range | 3 to 12 |
Material | Polypropylene |
Support Type | 6% Cross-linked Agarose |
Cytiva PD MidiTrap™ G-25 Sample Preparation Columns
Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 1mL. Cytiva Life Sciences™ PD MidiTrap™ G-25 Sample Preparation Columns are convenient and prepacked.
Cytiva His GraviTrap™ Columns
Use for purification of histidine-tagged proteins using gravity flow. His GraviTrap™ Columns enable fast and simple purification.
Cytiva HisTrap™ FF Columns: 5 x 5mL
Optimize purifications of histidine-tagged proteins directly from homogenized, unclarified cells HisTrap™ FF Columns: 5 x 5mL are prepacked with precharged Ni Sepharose™ 6 Fast Flow media.
Cytiva Ni Sepharose™ excel
Ni Sepharose excel affinity media for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
Particle Size | 90μm |
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Buffer | 0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol |
Flow Rate | 150 to 600cm/hr. |
Content And Storage | 4° to 30°C |
pH Range | 2 to 12 |
Product Type | Ni Sepharose excel chromato graphy media |
dimMatrix | Ni-Sepharose |
Support Type | 6% Highly Cross-linked Agarose |