Test
Gly-Phe-7-Amino-4-Trifluoromethylcoumarin, White powder
Product Type | Proteins, Enzymes & Peptides |
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Form | Powder |
Molecular Weight (g/mol) | 433.38 ; 574 (Including TFA salt) |
Color | White |
Storage Requirements | Keep cool and dry |
For Use With (Application) | Cell Migration Analysis; Cell Signaling Analysis |
Analog of the natural substrate
Detect beta-galactosidase expression from vectors, plasmids, or DNA fragments with this chromogenic substrate.
Hydrolysis of substrate yields brightly fluorescent product
The galactosidase substrate, DDAO galactoside yields a hydrolysis product that can be excited with the 633 nm laser (excitation/emission maxima ∼645/660).
PFB-FDGlu is a cell-permeable substrate used for live cell imaging of GCase activity. It fluoresces green upon cleavage by lysosomal GCase, facilitating specific, single-cell analysis via high-content imaging, flow cytometry, and microscopy.
Lyse cultured mammalian cells and measure beta-galactosidase activity using compatible M-PER Cell Lysis Reagent and colorimetric b-Gal Assay Reagent.
Substrate | Beta-Gal Substrate |
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Assay | Reporter Enzyme |
Substrate Properties | Chemical Substrate |
Content And Storage | Store at -20°C. |
Format | 96-well Plate |
Product Type | Pierce beta-Galactosidase Assay Reagent |
For Use With (Equipment) | Microplate Reader |
Form | Liquid |
Label or Dye | Enzyme Labeled |
Target Enzyme | Beta-Galactosidase |
For Use With (Application) | Absorbance |
Product Line | Pierce™ |
Detection Method | Colorimetric |
Use to assess cell viability as function of redox potential
Self-coating synthetic surface for stem cells
Novex™ AP Chemiluminescent Substrate (CDP-Star™)