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Real Time PCR Products

Real Time PCR Products

A range of products specifically designed for real-time PCR workflows, a process that monitors the amplification of a targeted DNA molecule during the PCR reaction, not at its end, as in conventional PCR. Products include assays, reagents, kits, and master mixes.
Real Time PCR Master Mixes (299)
Real Time PCR Assays and Arrays (298)
Real Time PCR Reagents and Kits (254)

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1 – 15 of 519 results
1

Applied Biosystems™ TaqMan™ Universal PCR Master Mix, no AmpErase™ UNG

TaqMan™ Universal PCR Master Mix, No AmpErase™ UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications.

Promotions are available
EDGE
Shipping Condition Wet Ice
Polymerase AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance High
Product Type Universal PCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
For Use With (Application) Gene Expression,Genotyping,miRNA analysis
PCR Method qPCR
Product Line TaqMan™
Detection Method Primer-probe
2

Applied Biosystems™ SYBR™ Green Universal Master Mix

Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.

Promotions are available
EDGE
Polymerase AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance High
Format Tube
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
Reaction Speed Standard
Label or Dye SYBR Green
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line SYBR™
Detection Method SYBR
3

Applied Biosystems™ TaqMan™ Universal PCR Master Mix

TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications.

EDGE
Polymerase AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance High
Product Type Universal PCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
For Use With (Application) Gene Expression,Genotyping,miRNA analysis
PCR Method qPCR
Product Line TaqMan™
Detection Method Primer-probe
4

Applied Biosystems™ MycoSEQ™ Mycoplasma Detection Kits

The MycoSEQ Mycoplasma Detection Kit is a real-time mycoplasma PCR assay designed and validated to meet the requirements of European Pharmacopeia Chapter 2.6.7 for use as a nucleic acid amplification technique (NAT).

EDGE
Description Detection for BioProduction process media, cells, raw materials
Label or Dye SYBR Green
Quantity 100 Reactions
For Use With (Application) Mycoplasma Detection
Product Line MycoSEQ™
5

Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (VIC™/MGB probe, primer limited)
GREENER_CHOICE

Eukaryotic 18S rRNA Endogenous Control (VIC™/MGB probe, primer limited)

EDGE
6

Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

EDGE
Shipping Condition Wet Ice
Polymerase Dual-Lock Taq DNA Polymerase
Format Tube
Product Type Real Time PCR SYBR Master Mix
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type DNA
Reaction Speed Fast or Standard
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line PowerUP™, SYBR™
Detection Method SYBR
7

TaqMan® TaqMan™ Gene Expression Assay (FAM)

Applied Biosystems TaqMan Gene Expression assays are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5' end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3' end.

Shipping Condition Room Temperature
GC-Rich PCR Performance High
Fidelity (vs. Taq) 60 X
Product Type Gene Expression Assay
Reaction Speed Fast
Green Features Sustainable packaging
PCR Method qPCR
Product Line TaqMan™
Detection Method Primer-probe
8

Applied Biosystems™ TaqMan™ Fast Advanced Master Mix for qPCR

TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in less time on an array of qPCR instrument platforms.

EDGE
Shipping Condition Wet Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Form Liquid
Concentration 2X
For Use With (Application) Gene Expression,miRNA analysis,Virus Detection
PCR Method qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Fast DNA Polymerase
Product Type Fast Advanced Master Mix
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 6 Pro, QuantStudio™ 7 Flex, QuantStudio™ 7 Pro, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast
Product Line TaqMan™
9

SYBR™ Power SYBR™ Green PCR Master Mix

Offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations.

EDGE
GC-Rich PCR Performance High
Purity or Quality Grade LD (Low DNA)
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Detection Method SYBR
Polymerase AmpliTaq Gold DNA Polymerase, AmpliTaq Gold LD
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7300 System, 7500 System, 7700 System, 7900HT Fast System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
Label or Dye SYBR Green
Product Line SYBR™
10

SYBR™ Fast SYBR™ Green Master Mix

Designed for speed, SYBR™ Green Master Mix provides a fast, reliable, and cost-effective solution for RT-PCR application without compromising sensitivity, specificity, dynamic range, or PCR efficiency.

EDGE
GC-Rich PCR Performance High
Purity or Quality Grade UP (Ultra Pure)
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Detection Method SYBR
Polymerase AmpliTaq Fast DNA Polymerase, AmpliTaq Fast UP
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast
Label or Dye SYBR Green
Product Line SYBR™
11

Applied Biosystems™ PowerTrack™ SYBR Green Master Mix for qPCR

PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up.

EDGE
Shipping Condition Dry Ice
Content And Storage • PowerTrack SYBR Green Master Mix
• 40X Yellow Sample Buffer

Store at -20±5°C in the dark.
Concentration 2X
For Use With (Application) Gene Expression,Virus Detection
PCR Method qPCR
Detection Method SYBR
Polymerase Taq DNA Polymerase
Format Tube
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, QuantStudio 12K Flex, Bio-Rad CFX Connect™, Bio-Rad CFX384 Touch™, Bio-Rad CFX96 Touch™, BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, ViiA™ 7 System, StepOne™ System, MJ Chromo4, MJ Opticon, StepOnePlus™ System, Roche LightCycler 480, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000
Sample Type DNA
Reaction Speed Fast or Standard
Product Line Applied Biosystems™
12

Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0

Accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors.

Purity or Quality Grade UP (Ultra Pure)
Concentration 2X
For Use With (Application) Gene Expression,Genotyping,Pathogen Detection,Virus Detection
PCR Method qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Format Bottle
Product Type qPCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOne™, Standard Mode
Sample Type Environmental Sample, Food
Reaction Speed Standard
Hot Start Built-In Hot Start
Product Line TaqMan™
13

TaqMan™ TaqMan™ Gene Expression Master Mix

Precise and reliable real-time qPCR quantification over 9 logs of linear dynamic range with Applied Biosystems™ TaqMan™ Gene Expression Master Mix.

EDGE
Polymerase AmpliTaq Gold DNA Polymerase
Purity or Quality Grade UP (Ultra Pure)
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR SystemStepOne™, Standard Mode, StepOnePlus™, Standard Mode
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line TaqMan™
Detection Method Primer-probe
14

Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG

TaqMan™ Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.

Promotions are available
EDGE
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Concentration 2X
For Use With (Application) Gene Expression,Genotyping,miRNA analysis
PCR Method qPCR
Detection Method Primer-probe
Polymerase AmpliTaq DNA Polymerase
Format Tube
Product Type Fast Universal PCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast
Product Line AmpErase™, TaqMan™
15

TaqMan™ TaqMan™ Advanced miRNA cDNA Synthesis Kit

Kit uses 3' poly-A tailing and 5' ligation of an adaptor sequence to extend the mature miRNAs present in the sample on each end prior to reverse transcription.