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Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.
Substrate Properties | Chemical Substrate |
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Product Type | ELISA Substrate |
Substrate Type | HRP (Horseradish Peroxidase) |
Detection Method | Colorimetric |
Stop TMB-based ELISA detection with this 0.16M sulfuric acid for use with the ELISA substrate 3,3',5,5' - tetramethylbenzidine (TMB).
HRP substrate optimized to generate an intense light signal and provide exception performance in luminometer-based assays.
Content And Storage | Upon receipt store product at room temperature. Product shipped at ambient temperature.Introduction |
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Substrate Properties | Chemical Substrate |
Product Type | ELISA Substrate |
Substrate Type | HRP (Horseradish Peroxidase) |
Product Line | SuperSignal™ |
Detection Method | Chemiluminescence |
Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.
Thermo Scientific™ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications.
Substrate Properties | Chemical Substrate |
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Product Type | ELISA Substrate |
Substrate Type | Alkaline Phosphatase |
Detection Method | Colorimetric |
Recommended for ELISA Ready-SET-Go!™ kits. eBioscience™ TMB ELISA Substrate Solution, 1x is a sensitive chromogenic substrate supplied as single ready-to-use solution.
Supplemented with fetal bovine serum as source of animal serum proteins. Used for blocking non-specific binding of ELISA plates, diluting recombinant standards and test samples and making the working dilutions of secondary detection reagent. Also contains 1% ProClin 150 as a preservative
Suitable for washing ELISA plate microwells. eBioscience™ ELISA Wash Buffer is supplied in 10 packets of concentrated powder, yielding a total of 10 liters of 1x Wash Buffer.
HRP substrate formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications.
Content And Storage | Stable for one year at 4°C or six months at room temperature. Product is shipped at ambient temperature. |
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Substrate Properties | Chemical Substrate |
Product Type | ELISA Substrate |
Substrate Type | HRP (Horseradish Peroxidase) |
Product Line | SuperSignal™ |
Detection Method | Chemiluminescence |
Ready-to-use, water-soluble peroxidase substrate tablets that yield a measurable green end-product for use in ELISA methods.
Preferred colorimetric beta-galactosidase (b-Gal) substrate for ELISA applications.
Enable sensitivity comparable to enhanced chemiluminescence (ECL) using the most sensitive fluorescent ELISA substrate for peroxidase (HRP).
Lysis buffer for total protein extraction from tissue samples
Concentrated wash buffer for efficient use of storage in the lab