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Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.
Thermo Scientific ATP (adenosine 5'-triphosphate) is an extremely stable nucleotide, and it is supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.
Specifically degrade single-stranded RNA at C and U residues with the endoribonuclease RNase A.
Stabilize enzymes, increase PCR yields, and prevent adhesion of enzymes with Thermo Scientific™ Bovine Serum Albumin.
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
Product Type | T4 DNA Ligase |
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Enzyme | DNA Ligase |
Compatible Buffer | 10X T4 DNA Ligase Buffer |
Concentration | 5 U/μL |
Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA).
Contains enzymes and buffer in a single mix to enable convenient ten-microliter reaction set-up with fewer pipetting steps.
Shipping Condition | Dry Ice |
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Content And Storage | Gateway™ LR Clonase™ II enzyme mix includes proteinase K solution (2 μg/μL) and a positive control vector. Store at -20°C or -80°C. Guaranteed stable for 6 months when properly stored. |
Product Type | LR Clonase Enzyme Mix |
Enzyme | LR Clonase |
Compatible Buffer | Enzyme Buffer |
Product Line | Clonase™, Gateway™ |
Degrade proteins even in the presence of detergents with Proteinase K, a broad-range endolytic protease widely used for digestion of proteins in nucleic acid preparations.
Form | Liquid |
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Product Type | Proteinase K |
RiboLock RNase Inhibitor specifically inhibits the activity of RNases A, B, and C, protecting RNA under a variety of reaction conditions.
Product Type | RNase Inhibitor |
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Compatible Buffer | Storage Buffer |
Concentration | 40 U/μL |
Product Line | RiboLock™ |
Catalyzes in vitro recombination of PCR products to generate entry clones
Shipping Condition | Dry Ice |
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Content And Storage | Gateway™ BP Clonase™ II enzyme mix includes proteinase K solution (2 μg/μl) and a positive control vector. Store at -20°C or -80°C. Guaranteed stable for 6 months when properly stored. |
Product Type | BP Clonase Enzyme Mix |
Enzyme | BP Clonase |
Compatible Buffer | Enzyme Buffer |
Product Line | Clonase™, Gateway™ |
Purified and tested for nondetectable levels of RNase contamination
Perform electrophoresis of nucleic acids in agarose and polyacrylamide gels using Thermo Scientific™ 10X TBE Buffer (Tris-borate-EDTA).
Shipping Condition | Dry Ice |
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Content And Storage | TURBO™ DNase and 10X Reaction Buffer should be stored at –20°C. |
Product Type | DNase |
Enzyme | DNase |
Compatible Buffer | 10X Reaction Buffer |
Concentration | 2 U/μL |
Product Line | Ambion™, TURBO™ |
Catalyze the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins using this thermosensitive alkaline phosphatase .
Product Type | Alkaline Phosphatase |
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Enzyme | Phosphatase |
Compatible Buffer | PCR Buffer, Enzyme Buffer |
Concentration | 1 U/μL |