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Research Category

Drug Development (4)
Genome Editing (2)
Scarless Cloning (62)
Traditional Cloning (458)

Sensitive to Heat Inactivation

No (68)
Yes (435)

Type IIS RE

No (432)
Yes (70)

Optimal Reaction Temperature

30°C (9)
37°C (443)
50°C (2)
55°C (21)
65°C (25)

Methylation Sensitivity

CpG Methylation-Sensitive (239)
Dam Methylation-Sensitive (37)
Dcm Methylation-Sensitive (22)
Not CpG Methylation-Sensitive (287)
Not Dam Methylation-Sensitive (456)
Not Dcm Methylation-Sensitive (443)

Detection Method

Biotin-based (1)
Fluorescence (49)
HRM (3)
Primer-probe (102)
Primer-probe Detection (1)
Radioactive, Fluorescence (1)
SYBR (39)

1 – 15 of 1,272 results

Thermo Scientific™ DH5α Competent Cells; High Efficiency

High efficiency, chemically competent E. coli cells ideal for construction of gene banks or generation of cDNA libraries using plasmid-derived vectors

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Pricing and Availability

Competent Cells

Invitrogen™ One Shot™ TOP10 Chemically Competent E. coli

One Shot TOP10 Chemically Competent E. coli are ideal for high-efficiency cloning and plasmid DNA propagation and are provided at a transformation efficiency of 1 x 10⁹ cfu/μg plasmid DNA.

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Pricing and Availability
Specifications

Competent Cells

Shipping Condition	Dry Ice
Blue/White Screening	Yes (lacZΔM15)
Cloning Methylated DNA	Yes (mcrA)
Transformation Efficiency Level	High Efficiency (>1 x 10⁹ cfu/μg)
High-throughput Compatibility	Low
Improves Plasmid Quality	Yes (endA1)
Preparing Unmethylated DNA	No
Plasmid	High Copy Plasmid
Reduces Recombination	Yes (recA1)
T1 Phage - Resistant (tonA)	No
Antibiotic Resistance Bacterial	Yes (Streptomycin)
Format	Tube
Product Type	Chemically Competent Cells
Species	E. coli (K12)
Product Line	One Shot™
Contains F' Episome	No

Thermo Scientific™ FastDigest EcoRI

The FastDigest EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer.

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Pricing and Availability
Specifications

Restriction Enzymes

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	EcoR I
Methylation Sensitivity	CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	5' protruding ends
Type IIS RE	No
Optimal Reaction Temperature	37°C
Inactivation Temperature	80°C
Product Line	FastDigest™

Invitrogen™ Lipofectamine™ RNAiMAX Transfection Reagent

Provides highest transfection efficiencies on widest variety of cell types for siRNA-mediated gene knockdown experiments

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Pricing and Availability
Specifications

Transfection Reagents

Shipping Condition	Approved for shipment at Room Temperature or on Wet Ice
Format	6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Product Type	Transfection Reagent
Transfection Technique	Lipid-based Transfection
Serum Compatible	Yes
Green Features	Sustainable packaging
High-throughput Compatibility	High-throughput Compatible
For Use With (Application)	Transfection
Product Line	Lipofectamine™
Cell Type	Established Cell Lines,Stem Cells,Primary Cells,Hard-to-Transfect Cells

Thermo Scientific™ T4 DNA Ligase (5 U/μL)

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.

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Pricing and Availability
Specifications

Modifying Enzymes

Product Type	T4 DNA Ligase
Enzyme	DNA Ligase
Compatible Buffer	10X T4 DNA Ligase Buffer
Concentration	5 U/μL

Thermo Scientific™ FastDigest XbaI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

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Pricing and Availability
Specifications

Restriction Enzymes

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Xba I
Methylation Sensitivity	Dam Methylation-Sensitive,Not CpG Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	5' protruding ends
Type IIS RE	No
Optimal Reaction Temperature	37°C
Inactivation Temperature	65°C
Product Line	FastDigest™

Invitrogen™ Gateway™ BP Clonase™ II Enzyme mix

Catalyzes in vitro recombination of PCR products to generate entry clones

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Pricing and Availability
Specifications

Modifying Enzymes

Shipping Condition	Dry Ice
Content And Storage	Gateway™ BP Clonase™ II enzyme mix includes proteinase K solution (2 μg/μl) and a positive control vector. Store at -20°C or -80°C. Guaranteed stable for 6 months when properly stored.
Product Type	BP Clonase Enzyme Mix
Enzyme	BP Clonase
Compatible Buffer	Enzyme Buffer
Product Line	Clonase™, Gateway™

Invitrogen™ Dynabeads™ M-280 Streptavidin

Dynabeads M-280 Streptavidin beads are 2.8-μm monosized, monodispersed, superparamagnetic, and hydrophobic beads covalently coupled to streptavidin.

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Pricing and Availability
Specifications

DNA Extraction and Purification

Shipping Condition	Ambient Temperature
Surface Functionality	Tosylactivated, Hydrophobic
Color	Brown
Binding Property	650-900 pmoles/mg beads
Concentration	10 mg/mL
High-throughput Compatibility	High-throughput Compatible
For Use With (Application)	Capture of Biotinylated Targets
Ligand Type	Streptavidin
Regulatory Status	For Research Use Only
Format	Beads in suspension
Bead Type	Magnetic Polystyrene
Product Type	Streptavidin Magnetic Beads
For Use With (Equipment)	KingFisher™ Sample Purification System, DynaMag™ magnets
Sample Type	Any Sample Type
Diameter (Metric)	2.8 μm
Certifications/Compliance	ISO9001 and ISO13485
Shelf Life	36 months from date of manufacture
Uniformity	Monosized 2.8 μm (CV <5%)
Product Line	Dynabeads™

Invitrogen™ SUPERase·In™ RNase Inhibitor (20 U/μL)

Binds and inactivates a wide variety of RNases

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Pricing and Availability
Specifications

RNA Preparation and Purification

Shipping Condition	Dry Ice
Concentration	50 mM
Product Line	Ambion™, SUPERase•In™

Thermo Scientific™ DH5α Competent Cells

Chemically competent E. coli cells recommended for routine subcloning into plasmid vectors. Subcloning efficiency cells are not suitable for the generation of cDNA libraries.

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Competent Cells

Thermo Scientific™ FastDigest BpiI (IIs class)

Cut at GAAGAC(2/6)^ sites with Thermo Scientific™ FastDigest™ BbsI (BpiI), which performs best using universal FastDigest Buffer (Isoschizomers: BpuAI, BstV2I).

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Restriction Enzymes

Thermo Scientific™ BpiI (BbsI) (10 U/μL)

Cut GAAGAC(2/6)^ sites with BpiI (BbsI) restriction enzyme, which performs best at 37°C in G buffer (Isoschizomers: BbsI, BpuAI, BstV2I).

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Pricing and Availability
Specifications

Restriction Enzymes

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	BpiI (BbsI)
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Scarless Cloning
Compatible Buffer	10X Buffer G
Type IIS RE	Yes
Optimal Reaction Temperature	37°C
Concentration	10 U/μL