Desalting Columns
Desalting Columns
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Thermo Scientific™ Zeba™ Spin Desalting Columns, Plates, and Cartridges, 7K MWCO, 0.5–100 mL
Perform high-throughput protein recovery (7K MWCO) and sample desalting for mass spectrometry, HPLC, capillary electrophoresis, metabolite screening, and assay development with ready-to-use Thermo Scientific Pierce Zeba desalting chromatography columns, plates, and cartridges.

MWCO | 7.0 kDa |
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Column Type | Size-exclusion, Proprietary Resin |
Purification Target | Buffer Exchange, Protein |
Product Line | Zeba™ |
Thermo Scientific™ Zeba™ Spin Desalting Columns and Plates, 40K MWCO, 75 μL–10 mL
The new and enhanced Zeba Spin and Micro Spin Desalting columns and plates are designed for efficient desalting and buffer exchanging proteins with a molecular weight >40 kDa and removing small molecules less than 2 kDa. They provide exceptional desalting and protein recovery performance.

Disposable | Single-use |
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MWCO | 40 kDa |
Column Type | Size-exclusion, Proprietary Resin |
Purification Target | Buffer Exchange, Protein |
Product Line | Zeba™ |
Cytiva Disposable PD-10 Desalting Columns
Use for desalting and buffer exchange, as well as removal of low-molecular weight compounds. Cytiva Life Sciences™ Disposable PD-10 Desalting Columns provide fast, high-recovery.

Thermo Scientific™ Zeba™ Dye and Biotin Removal Spin Columns and Filter Plates, 0.5–10 mL
Enable fast and efficient removal of non-reacted fluorescent dyes, biotin, reducing agents, and crosslinkers from protein samples with Thermo Scientific Pierce Zeba Dye and Biotin Removal columns and filter plates.

Shipping Condition | Room Temperature |
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Column Type | Proprietary Resin |
Product Line | Zeba™ |
Cytiva PD SpinTrap™ G-25 Desalting Columns
Use for convenient and fast small-scale cleanup of proteins and other large biomolecules (>5000 Mr). Cytiva Life Sciences™ PD SpinTrap™ G-25 Desalting Columns provide the option of running multiple samples in parallel.
Thermo Scientific™ Pierce™ Polyacrylamide Desalting Columns, 1.8K MWCO, 5 mL
Thermo Scientific Pierce 1.8K MWCO (molecular-weight cutoff) Polyacrylamide Desalting Columns are ready-to-use, disposable, gel-filtration columns for separating proteins and other macromolecules from low-molecular-weight buffer salts and reagents.
Cytiva HiTrap™ Desalting Prepacked Columns
For rapid convenient desalting and buffer exchange. HiTrap™ Desalting Prepacked Columns are prepacked, ready to use column for group separation between high and low molecular weight substances.
Disposable | Reusable |
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Particle Size | >20 ÎĽm (dry), 15 to 88 ÎĽm (wet) |
Content And Storage | 4°C to 30°C in 20% Ethanol |
Diameter (English) Inner Column | 0.62 in. |
Description | HiTrap Desalting are ready-to-use 5 mL columns prepacked with Sephadex G-25 Superfine resin for fast and convenient desalting and buffer exchange. |
Volume (Metric) Bed | 5 mL |
For Use With (Application) | For rapid convenient desalting and buffer exchange |
Diameter (Metric) Inner Column | 16 mm |
Thermo Scientific™ Pierce™ Dextran Desalting Columns (5K MWCO)
Ready-to-use, gel-filtration Thermo Scientific Pierce Dextran Desalting Columns can separate proteins and macromolecules from low-molecular weight buffer salts and reagents up to 5000 Daltons (5 kDa) in 5- or 10-mL volumes.
Disposable | Single-use |
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MWCO | 5.0 kDa |
Content And Storage | Store at room temperature or 4°C. |
Format | Gravity Flow Column |
Product Type | Desalting Column |
Column Type | Size-exclusion, Dextran Resin |
Quantity | 5 Columns |
Purification Target | Buffer Exchange, Protein |
Product Line | Pierce™ |
Cytiva™ MiniTrap™ Column
The Cytiva™ MiniTrap™ Columns are small-scale chromatography columns designed for rapid and convenient purification. They are commonly used for various applications including protein purification, antibody purification, peptide purification and nucleic acid purification.
Cytiva Disposable PD-10 Columns
Use for gravity flow purification. Disposable PD-10 Columns are economical, plastic columns that can be packed with a wide range of bulk media.
Cytiva HiPrep™ 26/10 Desalting Columns
Provide reliable and reproducible desalting and buffer exchange with sample sizes of up to 15mL per column. Cytiva HiPrep 26/10 Desalting Column is a convenient, easy-to-use column prepacked 53mL column with Sephadex G-25 Fine for desalting and buffer exchange.
Disposable | Reusable |
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Particle Size | >20 ÎĽm (dry), 17 to 132 ÎĽm (wet) |
Content And Storage | 4°C to 30°C in 20% Ethanol |
Diameter (English) Inner Column | 1.02 in. |
Description | HiPrep 26/10 Desalting are convenient, easy-to-use, 53 mL columns prepacked with Sephadex G-25 Fine resin for desalting and buffer exchange. |
Volume (Metric) Bed | 53 mL |
For Use With (Equipment) | Laboratory pump or chromatography system such as AKTA design |
Product Type | HiPrep 26/10 Desalting |
Diameter (Metric) Inner Column | 26 mm |
Thermo Scientific™ Pierce™ Polyacrylamide Spin Desalting Columns, 7K MWCO, 0.7 mL
Separate proteins and other macromolecules from low-molecular weight buffer salts and reagents with Thermo Scientific Pierce 7K MWCO (molecular-weight cutoff) Polyacrylamide Spin Desalting Columns, which are porous polyacrylamide-based gel-filtration columns for biological sample preparation.
MWCO | 7.0 kDa |
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Content And Storage | Upon receipt store at 4°C. |
Format | Spin Column |
Description | Pierce™ Polyacrylamide Spin Desalting Columns, 7K MWCO, 0.7 mL |
Product Type | Spin Desalting Column |
Column Type | Size-exclusion, Polyacrylamide Resin |
Purification Target | Buffer Exchange, Protein |
Sample Volume (Metric) | 30 to 120 ÎĽL |
Product Line | Pierce™ |
Cytiva Superdex 75 Increase Prepacked Column, 10/300 GL
Use these columns in preparative purification as well as for characterization and analysis of proteins with molecular weights between Mr 3,000 and 70,000, such as recombinant tagged proteins. Cytiva HisTrap™ excel Prepacked Columns feature tolerance to repeated harsh cleaning procedures at high pH ensures long column lifetime and minimizes carryover.
Cytiva Superdex 75 Increase Prepacked Column, 5/150 GL
Use this column for rapid purity check and screening of proteins with molecular weights between Mr 3,000 and 70,000, such as recombinant tagged proteins. Cytiva Superdex 75 Increase Prepacked Columns are ideal when time is limited and low buffer and sample consumption is important.
Cytiva Superdex 75 Increase Prepacked Column, 3.2/300
Use this column for rapid purity check and screening of proteins with molecular weights between Mr 3,000 and 70,000, such as recombinant tagged proteins. Cytiva Superdex 75 Increase Prepacked Columns are ideal when time is limited and low buffer and sample consumption is important.