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Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

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Specifications

Western Blot Substrates and Substrate Kits

Substrate	Horseradish Peroxidase
Form	Liquid
Product Type	Substrate
Signal Duration	6 to 8 hr.
Product Line	SuperSignal™
Detection Method	Chemiluminescence

Invitrogen™ Dynabeads™ Protein G for Immunoprecipitation

Dynabeads Protein G beads are uniform, 2.8-μm superparamagnetic beads with recombinant Protein G (∼17 kDa) covalently coupled to the surface.

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Specifications

Magnetic Affinity Separation Particles

Shipping Condition	Ambient temperature
Content And Storage	Dynabeads Protein G are supplied in PBS, pH 7.4 w/0.01% Tween-20, 0.09% NaN₃ Store at 2–8°C.
Purity or Quality Grade	For Research Use Only
Description	Recombinant Protein G covalently bound to Dynabeads
Surface Functionality	Protein G
Material	Polystyrene
Concentration	30 mg/mL
For Use With (Application)	Immunoprecipitation
High-throughput Compatibility	High-throughput Compatible
Ligand Type	Protein G
Type	Superparamagnetic Beads
Target	Antibodies
Format	Beads in suspension
For Use With (Equipment)	KingFisher™ Sample Purification System, DynaMag™ magnets
Diameter (Metric)	2.8 μm
Certifications/Compliance	ISO9001 and ISO13485
Uniformity	Monosized 2.8 mm (CV <5%)
Product Line	Dynabeads™

Thermo Scientific™ DTT (dithiothreitol)

Stabilize enzymes and other proteins which possess free sulfhydryl groups with DTT, shown to restore activity lost by oxidation of these groups in vitro.

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Specifications

Miscellaneous Molecular Biology Reagents

Product Type	DTT (Dithiothreitol)
Concentration	0.1 M

Invitrogen™ Dynabeads™ Protein A for Immunoprecipitation

Dynabeads Protein A beads are uniform, 2.8-μm superparamagnetic beads with recombinant Protein A (∼45 kDa) covalently coupled to the surface.

Pricing and Availability
Specifications

Magnetic Affinity Separation Particles

Shipping Condition	Ambient temperature
Content And Storage	Dynabeads Protein A are supplied in PBS, pH 7.4 w/0.01% Tween-20, 0.09% NaN₃ Store at 2–8°C.
Purity or Quality Grade	For Research Use Only
Description	Recombinant Protein A covalently bound to Dynabeads
Surface Functionality	Protein A
Material	Polystyrene
Concentration	30 mg/mL
For Use With (Application)	Immunoprecipitation
High-throughput Compatibility	High-throughput Compatible
Ligand Type	Protein A
Type	Superparamagnetic Beads
Target	Antibodies
Format	Beads in suspension
For Use With (Equipment)	KingFisher™ Sample Purification System, DynaMag™ magnets
Diameter (Metric)	2.8 μm
Certifications/Compliance	ISO9001 and ISO13485
Uniformity	Monosized 2.8 mm (CV <5%)
Product Line	Dynabeads™

Invitrogen™ Qubit™ Protein and Protein Broad Range (BR) Assay Kits

Achieve sensitive detection of protein, and low protein-to-protein variation, with the Qubit Protein and Protein BR Assay Kits for fluorescence-based protein quantitation assays.

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Specifications

Total Protein Assay Kits

Product Line	Qubit™
Detection Method	Fluorescence

Thermo Scientific™ RNA Gel Loading Dye (2X)

Prepare RiboRuler RNA ladders and RNA samples for electrophoresis on agarose or polyacrylamide gels using Thermo Scientific™ 2X RNA Loading Dye.

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Miscellaneous Molecular Biology Reagents

Invitrogen™ Alexa Fluor™ 647 NHS Ester (Succinimidyl Ester)

Alexa Fluor™ 647 is a bright and photostable far-red dye with excitation ideally suited to the 633 nm laser line.

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Specifications

Protein Labeling Reagents

Emission	672 nm
Excitation	651 nm

Gibco™ Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation

Dynabeads Human T-Activator CD3/CD28 beads are 4.5-μm superparamagnetic beads covalently coupled with an optimized combination of anti-human CD3 and anti-human CD28 antibodies and intended for ex vivo activation and expansion/proliferation of human T cells.

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Specifications

Microspheres

Shipping Condition	Ambient Temperature
Clonality	Monoclonal
Target Species	Human T-cells
Host Species	Mouse
Surface Functionality	Epoxy
Isolation Technology	Magnetic Beads
Material	Magnetic Polystyrene
Concentration	4 x 10 ⁷ Beads/mL
For Use With (Application)	Activation and Expansion of T-cells
Regulatory Status	For Research Use Only
Reactivity	Human
For Use With (Equipment)	DynaMag™ Magnet
Product Type	Antibody Coated Bead
Diameter (Metric)	4.5 μm
Shelf Life	24 months from date of manufacture
Certifications/Compliance	ISO9001 and ISO13485
Uniformity	Monosized 4.5 μm (CV <5%)
Product Line	DynaBeads™

Thermo Scientific™ EDTA (0.5 M), pH 8.0

Optimize biochemistry or molecular biology applications that require a chelator for divalent cations.

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Miscellaneous Molecular Biology Reagents

Thermo Scientific™ X-Gal

Effectively screen colonies with X-Gal, an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal, forming an intense blue precipitate.

Pricing and Availability
Specifications

Miscellaneous Molecular Biology Reagents

Product Type	X-Gal

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

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Pricing and Availability
Specifications

Western Blot Substrates and Substrate Kits

Substrate	Horseradish Peroxidase
Content And Storage	Upon receipt store at 2-8°C. Product is shipped at ambient temperature.
Form	Liquid
Product Type	Substrate
Signal Duration	1 to 2 hr.
Product Line	Pierce™
Detection Method	Chemiluminescence

Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate

Luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.

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Pricing and Availability
Specifications

Western Blot Substrates and Substrate Kits

Substrate	Horseradish Peroxidase
Sensitivity	Mid-femtogram
Form	Liquid
Product Type	Substrate
Signal Duration	24 hr.
Product Line	SuperSignal™
Detection Method	Chemiluminescence

Gibco™ Human EGF, Animal-Free Recombinant Protein, PeproTech®

Recombinant Protein

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Pricing and Availability
Specifications

Recombinant Proteins

Content And Storage	-20°C
Purity or Quality Grade	≥ 98% by SDS-PAGE gel and HPLC analyses.
Conjugate	Unconjugated
Form	Lyophilized
Common Name	EGF
Molecular Weight (g/mol)	6.2 kDa
Gene Symbol	EGF
Endotoxin Concentration	<0.1 EU/ μg
Sequence	NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR
Expression System	E. coli
For Use With (Application)	Functional Assay
Source	E. coli
Name	Human EGF, Animal-Free
Accession Number	P01133
Regulatory Status	RUO
Gene Alias	AI790464; beta-urogastrone; EGF; Epidermal growth factor; epidermal growth factor (beta-urogastrone); epidermal growth factor precursor; H-EGF; HOMG4; Pro-epidermal growth factor; Pro-epidermal growth factor precursor (EGF); sb:eu639; URG; Urogastrone
Biological Activity	The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 10^7 units/mg
Gene ID (Entrez)	1950
Formulation	protein with no preservative
Recombinant	Recombinant

Thermo Scientific™ Slide-A-Lyzer™ G3 Dialysis Cassettes, 3.5K MWCO

Thermo Scientific Slide-A-Lyzer™ G3 Dialysis Cassettes facilitate the rapid and trouble-free dialysis of sample volumes from 0.5 to 125 mL. Unlike standard flat tubing, these innovative devices do not require knots or clips that can lead to leaking and sample loss.

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Pricing and Availability
Specifications

Dialysis Cassettes

MWCO	3.5 kDa
Format	Cassette
Membrane	Cellulose Membrane, Dialysis
Green Features	Less waste
Purification Target	Buffer Exchange, Protein
Product Line	Slide-A-Lyzer™

Thermo Scientific™ PVDF Transfer Membrane, 0.2μm, 26.5 cm x 3.75 m

PVDF Transfer Membrane, 0.2 μm, 26.5 cm x 3.75 m

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Western Blot and Nucleic Acid Membranes and Filter Paper

Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate

Invitrogen™ Dynabeads™ Protein G for Immunoprecipitation

Thermo Scientific™ DTT (dithiothreitol)

Invitrogen™ Dynabeads™ Protein A for Immunoprecipitation

Invitrogen™ Qubit™ Protein and Protein Broad Range (BR) Assay Kits

Thermo Scientific™ RNA Gel Loading Dye (2X)

Invitrogen™ Alexa Fluor™ 647 NHS Ester (Succinimidyl Ester)

Gibco™ Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation

Thermo Scientific™ EDTA (0.5 M), pH 8.0

Thermo Scientific™ X-Gal

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate

Gibco™ Human EGF, Animal-Free Recombinant Protein, PeproTech®

Thermo Scientific™ Slide-A-Lyzer™ G3 Dialysis Cassettes, 3.5K MWCO

Thermo Scientific™ PVDF Transfer Membrane, 0.2μm, 26.5 cm x 3.75 m

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Invitrogen™ Qubit™ Protein and Protein Broad Range (BR) Assay Kits Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Thermo Scientific™ Slide-A-Lyzer™ G3 Dialysis Cassettes, 3.5K MWCO Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

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Invitrogen™ Qubit™ Protein and Protein Broad Range (BR) Assay Kits

Thermo Scientific™ Slide-A-Lyzer™ G3 Dialysis Cassettes, 3.5K MWCO