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Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG

TaqMan™ Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.

Brand: Applied Biosystems™ 4366073

6770.00 DKK valid until 2025-04-18
Use promo code "24107" to get your promotional price.

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Product Code. 10575385

13540.00 DKK / 1250 reactions

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Requires : Template and primers
This item is not returnable. View return policy

Description

Results in 40 minutes for 40 cycles of PCR in a 20 μL reaction volume when using the Applied Biosystems 7900HT and Fast 7500 Fast real-time PCR systems
The optimized formulation contains AmpliTaq Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and allowing room temperature reaction setup
A proprietary ROX dye serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations, for superb precision on Applied Biosystems real-time PCR instruments
Contains everything needed for TaqMan-based qPCR amplification and detection in a single-tube format
TaqMan Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR
Compatible with TaqMan Gene Expression assays
Store at 2°C to 8°C

Order Info

Guaranteed minimum shelf life is 60 days.

Specifications

Content And Storage	This 5-pack contains five kits, each containing 2 tubes (1.25 ml each) of 2X TaqMan™ Fast Universal PCR Master Mix. Store at 2°C to 8°C. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Format	Tube
PCR Method	qPCR
Reaction Speed	Fast
Passive Reference Dye	ROX (Pre-mixed)
Product Type	Fast Universal PCR Master Mix
Sufficient For	250 Reactions
Concentration	2X
No. of Reactions	1250 Reactions
Volume	1.25 mL

Detection Method	Primer-probe
GC-Rich PCR Performance	High
Polymerase	AmpliTaq DNA Polymerase
For Use With (Equipment)	7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Product Line	AmpErase™, TaqMan™
Quantity	1,250 reactions
For Use With (Application)	Gene Expression, Genotyping, miRNA analysis
Fidelity (vs. Taq)	2 X
Sample Type	DNA (Genomic), cDNA

Produktvorschläge

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Dokumentation

Product Information

Manuals

Manual: Performing Fast Gene Quantitation with 384-Well Plates: Applied Biosystems 7900HT Fast Real-Time PCR System: User Bulletin (English ) Version: 20 Jun 2007 File size: 254.8 kB

Product Sheet: TaqMan Fast Universal PCR Master Mix, no AmpErase UNG Version: E File size: 132.7 kB

Manual: Real-Time PCR Systems (English ) Version: 25 Jul 2007 File size: 1.7 MB

Product Info Sheet: TaqMan RNase P Instrument Verification Plate, 384-well Version: 4323307 Rev.E (01Dec2015) File size: 178.1 kB

Warranties

Brochures & Specifications: Applied Biosystems TaqMan® Fast Universal PCR Master Mix: Product Bulletin (English ) File size: 319.8 kB

Scientific Resources

Application Notes

Application Tutorial: Applied Biosystems Real-Time PCR Rapid Assay Development Guidelines File size: 243.7 kB

Brochures

Brochures & Specifications: Comparing Fast and Standard Data on Applied Biosystems 7500 and 7500 Fast Real-Time PCR Systems: Data Sheet (English ) File size: 273.7 kB

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