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Applied Biosystems™ MeltDoctor™ HRM Reagent Kit

Provides individually packaged PCR components and the MeltDoctor™ HRM Dye required for high resolution melting analysis.

Brand: Applied Biosystems™ 4425557

Product Code. 10648095

6130.00 DKK / 1 set

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Includes: AmpliTaq Gold™ 360 DNA Polymerase, AmpliTaq Gold 360 Buffer, 360 GC Enhancer, GeneAmp™ dNTP Blend, MeltDoctor HRM Dye (20X)

This item is not returnable. View return policy

Description

High Resolution Melting (HRM) analysis is a post-PCR analysis method used to identify genetic variation in nucleic acid sequences.

This kit includes:

AmpliTaq Gold™ 360 DNA Polymerase which, when combined with AmpliTaq Gold™ 360 Buffer and 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. AmpliTaq Gold™ 360 DNA Polymerase delivers 360° coverage for a full range of targets
GeneAmp™ dNTP Blend
MeltDoctorHRM Dye, a stabilized form of the fluorescent SYTO™ 9 double-stranded nucleic acid stain developed by Molecular Probes

Gene Expression Analysis & Genotyping, Genetic Variation Analysis Using Real-Time PCR, Genotyping & Genomic Profiling, High Resolution Melting (HRM) Analysis, Methylation Analysis, Methylation-Sensitive HRM Analysis, PCR & Real-Time PCR, RNAi, Epigenetics & Noncoding RNA Research, Real Time PCR (qPCR)

Order Info

Guaranteed minimum shelf life is 60 days.

Specifications

Content And Storage	This kit includes: AmpliTaq Gold™ 360 DNA Polymerase (2 x 50 μL, each at 5 U/μL), AmpliTaq Gold™ 360 Buffer, 360 GC Enhancer, GeneAmp™ dNTP Blend, and MeltDoctor™ HRM Dye (20x). The kit provides sufficient materials for 250 20-μL reactions when using 2 U AmpliTaq Gold 360 DNA Polymerase per reaction. Store at -15° to -25° Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
GC-Rich PCR Performance	High
Polymerase	AmpliTaq Gold 360 DNA Polymerase
For Use With (Equipment)	7500 Fast System, 7900HT Fast System
Label or Dye	High Resolution Melting (HRM), SYTO 9
Product Type	Hrm Reagent Kit
For Use With (Application)	Real Time PCR (qPCR)

Detection Method	HRM
PCR Method	qPCR
Technique	HRM (High Resolution Melting) Analysis, Mutation Scanning
Genotyping Target	SNPs (Unknown or Numerous), Epigenetic Targets
Product Line	MeltDoctor™
Quantity	1 kit
Sample Type	DNA (Genomic), cDNA

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Applied Biosystems™ MeltDoctor™ HRM Reagent Kit

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