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Applied Biosystems™ TaqMan™ Universal Master Mix II, no UNG

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).

Brand: Applied Biosystems™ 4440040

2112.50 DKK valid until 2025-06-30
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Product Code. 10380155

4225.00 DKK / Each

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Requires : Primers, probes, template, water
This item is not returnable. View return policy

Description

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR). Use TaqMan Universal Master Mix II to amplify complementary DNA (cDNA) and DNA targets for a variety of applications, including quantitation and genotyping.

This TaqMan Universal Master Mix II is supplied as a 2X concentrate and contains:
• AmpliTaq Gold DNA Polymerase, UP (Ultra Pure)
• dNTPs (with dUTP)
• ROX Passive Reference
• Optimized buffer components

Note: This TaqMan Universal Master Mix II does not contain uracil-N-glycoslyase (UNG).

TaqMan Universal Master Mix II has been optimized for use with primers and TaqMan probes that have been designed according to Thermo Fisher Scientific guidelines. The master mix can be used with custom TaqMan assays available from our custom assay service or with pre-optimized assays, such as:
• TaqMan gene expression assays
• TaqMan microRNA assays
• TaqMan drug metabolism genotyping assays
• TaqMan SNP genotyping Assays

For RNA quantitation experiments, TaqMan Universal Master Mix II is used in the second step of a two-step reverse transcription/polymerase chain reaction (RT-PCR) protocol. The cDNA template used with the master mix can be generated in a reverse transcription reaction using cDNA synthesis kits available from Thermo Fisher Scientific.

Alternative product: Try TaqMan Fast Advanced Master Mix (Cat. No. 4444963), our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Universal Master Mix II and added additional capabilities for your gene expression analysis.

Order Info

Guaranteed minimum shelf life is 60 days. Shipping Conditions: Wet Ice.

Specifications

Content And Storage	Store at 2°C to 8°C in the dark. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
GC-Rich PCR Performance	High
Polymerase	AmpliTaq Gold DNA Polymerase
For Use With (Equipment)	7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Product Line	TaqMan™
Purity or Quality Grade	UP (Ultra Pure)
Shipping Condition	Wet Ice
Concentration	2X
Sample Type	DNA (Genomic), cDNA

Detection Method	Primer-probe
PCR Method	qPCR
Reaction Speed	Standard
Passive Reference Dye	ROX (Pre-mixed)
Product Type	Universal Master Mix II
Quantity	1 x 5 mL
For Use With (Application)	Gene Expression, Genotyping, miRNA analysis
Fidelity (vs. Taq)	2 X
Volume	5 mL

Product Suggestions Documents

Documents

Product Information

Manuals

Protocol: TaqMan® Universal Master Mix II Quick Reference Card (English ) Version: 08 Jul 2009 File size: 83.1 kB

Product Bulletin: TaqMan® Universal Master Mix II Version: 2 File size: 1.1 MB

Product Sheet: TaqMan Universal Master Mix II Version: C File size: 133.6 kB

Manual: TaqMan Universal Master Mix II Quick Reference Guide(Japanese) Version: 4428174A 07.2009 File size: 244.3 kB

Specification Sheets

Brochures & Specifications: TaqMan® Universal Master Mix II File size: 524.4 kB

Certificates

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number2791674	Certificate TypeCertificate of Analysis	Date31/01/2025	Catalog Number4440040,4440040_LITL18 PROMO
Lot Number2748832	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog Number4440040,4440040_LITL18 PROMO
Lot Number2805007	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog Number4440040,4440040_LITL18 PROMO
Lot Number2822266	Certificate TypeCertificate of Analysis	Date28/01/2025	Catalog Number4440040,4440040_LITL18 PROMO
Lot Number2907899	Certificate TypeCertificate of Analysis	Date27/01/2025	Catalog Number4440040,4440040_LITL18 PROMO

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