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Thermo Scientific™ beta-Galactosidase Assay Reagent
Lyse cultured mammalian cells and measure beta-galactosidase activity using compatible M-PER Cell Lysis Reagent and colorimetric b-Gal Assay Reagent.
1190.00 DKK - 2630.00 DKK
Specifications
| Assay | Reporter Enzyme |
|---|---|
| Content And Storage | Store at -20°C. |
| Detection Method | Colorimetric |
| Form | Liquid |
| For Use With (Application) | Absorbance |
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
|
10638844
|
Thermo Scientific™
75705 |
25 mL |
1190.00 DKK
25mL |
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|
11849420
|
Thermo Scientific™
75710 |
3 x 25 mL |
2630.00 DKK
Pack of 3 |
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Description
Perform a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal activity using Thermo Scientific™ Mammalian β-Galactosidase Assay Kit.
The Assay Reagent and Kit comprise a simple and versatile assay system for measuring β-galactosidase activity. Mammalian cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single, all-in-one lysis-and-assay solution. Alternatively, the included Thermo Scientific M-PER™ Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to quantitate the β-Gal activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and Tyrosine Kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.Highlights:
- Choose stand-alone assay reagent or complete kit for cell lysis and detection
- Colorimetric assay easily measured in a plate reader or spectrophotometer (405nm)
- Adaptable to 96-well microplates or tube assay formats
- Perform homogeneous screening assays or measure activity secondarily after cell lysis
- Also compatiible with prepared lysates from other species such as bacteria
Includes:
Stand-alone Thermo Scientific Pierce™ β-Galactosidase Assay Reagent, Thermo Scientific M-PER Mammalian Protein Extraction Reagent (Part No. 78503), and ready-to-use Stop Solution
Specifications
| Reporter Enzyme | |
| Colorimetric | |
| Absorbance | |
| Beta-Gal Substrate | |
| Beta-Galactosidase | |
| Microplate Reader | |
| Pierce beta-Galactosidase Assay Reagent |
| Store at -20°C. | |
| Liquid | |
| Pierce™ | |
| Chemical Substrate | |
| 96-well Plate | |
| Enzyme Labeled |
Safety and Handling
Product Identifier
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Pierce Beta-Galactosidase Assay Reagent
Hazard Category
- LONG-TERM AQUATIC HAZARD Chronic 3
Hazard Statement
- H412-Harmful to aquatic life with long lasting effects.
Precautionary Statement
- P273-Avoid release to the environment.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : 2-Mercaptoethanol
For Research Use Only. Not for use in diagnostic procedures.
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Product Title
Thermo Scientific™ beta-Galactosidase Assay Reagent