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Thermo Scientific™ TNBSA Solution (2,4,6-trinitrobenzene sulfonic acid) (5% w/v)
Label primary amines (peptides or amino acids) for colorimetric detection (Amax 335nm) using this 5% solution of trinitrobenzene sulfonic acid in methanol.
Brand: Thermo Scientific™ TS-28997
This item is not returnable.
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Description
Thermo Scientific™ Pierce TNBSA is a 5% solution of trinitrobenzene sulfonic acid in methanol that reacts with primary amines (peptides or amino acids) to yield a soluble colored product, a property useful for various assay methods.
Highlights:
- Couples with primary amines, sulfhydryls and hydrazides in aqueous solution at pH 8, without undesirable side reactions
- Excellent for solution or solid phase analysis
- Suitable for qualitative and quantitative estimation of biomolecules
- λmax = 335nm
- Reacts readily with primary amino groups of amino acids in aqueous format at pH 8 to form yellow adducts
- No colored derivatives are formed with secondary amino acids proline and hydroxyproline
- Colored derivatives are monitored at 345nm and have extinction coefficients in range of 1-1.5 x 104
Specifications
| TNBSA Solution | |
| Storage: Upon receipt store at -20°C. Product is shipped at ambient temperature. | |
| Colorimetric | |
| Liquid |
| Amine | |
| Other Label or Dye | |
| 100 mL |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- TNBSA Solution (2,4,6-trinitrobenzene sulphonic acid)
Signal Word
- Danger
Hazard Category
- Acute toxicity Category 3
- Serious eye damage/eye irritation Category 1
- Flammable liquid Category 2
- Skin corrosion/irritation Category 1 A
- Skin sensitiser Category 1
- Specific target organ toxicity after single exposure Category 1
Hazard Statement
- H225-Highly flammable liquid and vapour.
- H301-Toxic if swallowed.
- H311-Toxic in contact with skin.
- H314-Causes severe skin burns and eye damage.
- H317-May cause an allergic skin reaction.
- H331-Toxic if inhaled.
- H370-Causes damage to organs.
Precautionary Statement
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P370+P378-In case of fire: Use dry sand, dry chemical, or alcohol-resistant foam to extinguish.
- P403+P235-Store in a well-ventilated place. Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : 2,4,6-trinitrobenzenesulphonic acid, methyl alcohol
Limited warranty period: Subject to all warranty requirements and exclusions set out in our Terms and Conditions of Sale, this product is warranted from the date we ship the product and for ninety (90) days thereafter. Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.