Thermo Scientific™ SureTect™ Brettanomyces spp. and Saccharomyces cerevisiae Multiplex PCR Assay

Description

Brettanomyces is responsible for the presence of 4-ethyl phenol in wine, juice and high sugar beverages. The presence of Brettanomyces may affect S. cerevisiae fermentation and can impact the color, odor and taste of the produced beverages, leading to bottle recalls, production shut down, damage to brand reputation and loss in consumers' confidence. Maintaining the high quality of each product starts with regular monitoring of environment and early detection in semi-finished and finished products for a rapid cleaning action.

Designed specifically for control programs in beverage production, the Thermo Scientific™ SureTect™ Brettanomyces spp. and S. cerevisiae Multiplex PCR Assay rapidly detects and differentiates both targets using a simple and optimized workflow. The assay has been extensively tested and verified to address the process challenges by offering accuracy, highly-specific results and efficiency in environment monitoring and product release.

The SureTect™ Brettanomyces spp. and S. cerevisiae Multiplex PCR Assay is an optimized solution combining speed and performance in an easy-to-use and cost-effective platform, giving robust results that you can always trust.

Simple

• Easy-to-use, simple workflow
• Pre-dispensed lysis reagents and PCR lyophilized reagents
• Automatic lysis cycle with very few pipetting steps

Fast and Flexible

• Fast screening and differentiation of Brettanomyces and S. cerevisiae in a single PCR test
• Rapid detection for early actions and high confidence in production
• Test up to 96 samples in each run
• Choice of pierceable seals or caps
• Open PCR platforms

Smart

• Highly specific assay that covers the 3 main species of Brettanomyces: B. naardenensis, B. anomalus and B. bruxellensis
• Multiplex PCR to reduce cost and hands-on time
• Optimized workflow with 3 pierceable seals to reduce cross-contamination
• Intuitive, user-friendly software delivers reliable results, with no subjective interpretation
• Actionable data supports fast decisions in a complex product supply chain
• Internal positive control (IPC) in every assay for complete confidence in results

The SureTect™ Brettanomyces spp. and S. cerevisiae Multiplex PCR Assay Kit includes:

• Lysis Tubes
• Lysis Reagent 3
• Lysis Reagent 3 Buffer
• SureTect Brettanomyces and S. cerevisiae PCR Assay Tubes
• Thermo Scientific 96-well Pierceable Seals (3 seals per kit)
• SureTect PCR Caps

The SureTect™ Brettanomyces spp. and S. cerevisiae Multiplex PCR Assay is validated on Applied Biosystems™ QuantStudio™ 5 Food Safety Real-Time PCR System with RapidFinder™ Analysis Software v3.0 or later, allowing you to test easily and confidently. After completing the run, interpreted results are clearly displayed and can be reported, stored, printed or downloaded as required.