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The Amplex Red and UltraRed reagents are fluorogenic substrates for peroxidase. These highly sensitive and stable probes react with H2O2 in the presence of HRP for solution-based assays and ELISAs. Amplex UltraRed is a brighter version of Amplex Red with an improved pH range and sensitivity.
Substrate | HRP (Horseradish Peroxidase) Substrate |
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Shipping Condition | Room Temperature |
Content And Storage | Store in freezer (-5°C to -30°C). |
Form | Solid |
Product Type | Red Hydrogen Peroxide |
For Use With (Equipment) | Fluorometer,Microplate Reader |
Validated Application | Cell Analysis |
For Use With (Application) | Hydrogen Peroxidase Assay |
Product Line | Amplex™ |
Detection Method | Fluorescence |
PFB-FDGlu is a cell-permeable substrate used for live cell imaging of GCase activity. It fluoresces green upon cleavage by lysosomal GCase, facilitating specific, single-cell analysis via high-content imaging, flow cytometry, and microscopy.
Produces a green fluorescence
Analog of the natural substrate
Use to assess cell viability as function of redox potential
Detect beta-galactosidase expression from vectors, plasmids, or DNA fragments with this chromogenic substrate.
The fluorogenic DQ™ collagen can be used to directly monitor collagenase activity.
Hydrolysis of substrate yields brightly fluorescent product
MUP is a fluorescent substrate for alkaline phosphatases
Continuously assay acid phosphatases at low pH
Shipping Condition | Room Temperature |
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Excitation/Emission | 358⁄455 |
Substrate | Phosphatase Substrate |
Substrate Properties | Chemical Substrate |
Content And Storage | Store in freezer (-5 to -30°C). |
For Use With (Equipment) | Microplate Reader |
Form | Powder |
Label or Dye | DiFMU |
Color | Blue |
Target Enzyme | Phosphatases |
Detection Method | Fluorescence |
Suited for antigen processing and presentation studies