Protein Purification
Protein Purification
Cytiva HisTrap™ HP Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
Cytiva Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns
For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.
Cytiva Superose™ 6 Increase Columns
High-resolution size-exclusion chromatography for studying large proteins and complexes. Cytiva Lifescience™ Superose™ 6 Increase Columns delivers high quality in size exclusion chromatography (SEC).
Cytiva HisTrap™ FF Columns: 16 x 25mm
Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.
Cytiva PD MiniTrap™ G-25 Sample Preparation Columns
Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.
Thermo Scientific™ HisPur™ Ni-NTA Resin
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
Promotions are available
Invitrogen™ DynaMag™-2 Magnet
The DynaMag-2 magnet combines a strong magnetic attraction for efficient separations in standard microcentrifuge tubes with a flexible and smart ergonomic design.
Promotions are available
Cytiva Gel Filtration Calibration Kits
Calibrate gel filtration columns simply, reliably. Cytiva Life Sciences™ Gel Filtration Calibration Kits contain a well-defined series of protein standards.
Thermo Scientific™ Pierce™ Protease Inhibitor Mini Tablets, EDTA-free
Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments
Promotions are available
Thermo Scientific™ Lysozyme
Degrade unwanted bacterial cell wall in protein or nucleic acid extracts
Promotions are available
Thermo Scientific™ UltraLink™ Hydrazide Resin
Immobilize polyclonal antibodies or other glycoproteins, via periodate-oxidized sugars, onto hydrazide-activated polyacrylamide beads for AP, IP or pull-down assays.
Thermo Scientific™ Column Extenders for Pierce™ Centrifuge Columns
Pack these empty 0.5 to 10mL spin columns with agarose beads to perform small-scale affinity purification methods in microcentrifuge, 15mL conical or 50mL conical tubes.
Invitrogen™ DynaMag™- Spin Magnet
DynaMag-Spin magnet is optimized for efficient magnetic separation of all types of Dynabeads (range 1–4.5 μm in diameter) in small sample volumes (1.5 mL) in microcentrifuge tubes.
Thermo Scientific™ Column Funnels for Pierce™ Disposable Columns
Empty drip columns (0.5 to 5 mL) with matching frits and caps for packing beaded affinity or desalting resins to use in gravity-flow purification techniques.
Thermo Scientific™ Pierce™ Chromogenic Endotoxin Quant Kit
Chromogenic Endotoxin Quant Kits are highly sensitive endpoint assays that measure and detect endotoxin (LPS) in protein, peptide, nucleic acid, or antibody samples using the Amebocyte Lysate Assay from 0.01–1.0 EU/mL. Kits are available in reaction sizes ranging from 30 to 240.
Promotions are available
