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Applied Biosystems™ TaqMan™ MicroRNA Assay
Applied Biosystems TaqMan MicroRNA Assays quantitate miRNAs with the specificity and sensitivity of TaqMan Assay chemistry. A simple, two-step protocol requires only reverse transcription with an miRNA-specific primer, followed by real-time PCR with TaqMan probes.
Brand: Applied Biosystems™ 4440887
This item is not returnable.
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Description
Applied Biosystems TaqMan MicroRNA Assays quantitate miRNAs with the specificity and sensitivity of TaqMan Assay chemistry. A simple, two-step protocol requires only reverse transcription with an miRNA-specific primer, followed by real-time PCR with TaqMan probes. TaqMan MicroRNA Assays are:
• Highly specific—quantitate only mature miRNAs, not precursors
• Sensitive—conserve limited samples, requires only 1–10 nanograms of total RNA or equivalent
• Fast, simple and scalable—two-step quantitative RT-PCR assay provides high-quality results in less than three hours
Our TaqMan MicroRNA Assay catalogue is kept up to date with the Sanger database to provide comprehensive coverage with the most current annotation.
Availability and sizes
Made-to-Order assays are available in four different sizes and usually ship within two weeks. Inventoried assays are prepackaged in Small size and are ready to ship. Inventoried assays may be purchased in larger sizes by ordering them as Made to Order.
Species
TaqMan MicroRNA Assays are available for a range of species including human, mouse, rat, Drosophila, C. elegans, and Arabidopsis. We will continue to increase the number of miRNA assays for these species in alignment with the Sanger miRBase Registry.
Assay Components
TaqMan MicroRNA Assays are delivered in two tubes: one containing the RT primer, the other containing the specific pre-formulated TaqMan Assay (TaqMan probe and PCR primer set).
Pre-formulated assay
• Forward primer: 1500 nM
• Reverse primer: 700 nM
• FAM-MGB probe: 200 nM
• RT sequences: 50 nM
TaqMan miRNA Assay selection guide
TaqMan MicroRNA Assays
Description: TaqMan MicroRNA Assays employ a novel target-specific stem–loop primer during cDNA synthesis to produce a template for real-time PCR
RT chemistry: miRNA-specific RT
Throughput: Best for 1–10 targets
Coverage: 205 species available, coverage for miRBase v.21
TaqMan Advanced miRNA Assays
Description: TaqMan Advanced miRNA Assays employ a universal RT step for a streamlined workflow, and a universal miR-Amp step to enable highly sensitive detection by real-time PCR
RT chemistry: Universal RT
Throughput: Best for >10 targets
Coverage: All human, mouse, and rat miRNAs; coverage for miRBase v.24
Specifications
| • 1 tube containing RT primer at concentration of 5X (XS and S sizes), 20X (M size), or 60X (L size) • 1 tube containing a 20X (XS, S, and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers). Store at -15 to -25°C. |
|
| Tube | |
| qPCR | |
| 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex | |
| TaqMan™ | |
| Room Temperature | |
| 750 Reactions |
| M (750 RT/750 PCR reactions), Made to Order | |
| High | |
| Fast | |
| Sustainable packaging | |
| qPCR Reagents | |
| 750 Reactions |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- TaqMan MicroRNA Assay
Signal Word
- Danger
Hazard Category
- Carcinogenicity Category 2
- Reproductive toxicity Category 1B
Hazard Statement
- H351-Suspected of causing cancer.
- H360-May damage fertility or the unborn child.
Precautionary Statement
- P201-Obtain special instructions before use.
- P202-Do not handle until all safety precautions have been read and understood.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Formamide
For Research Use Only. Not for use in diagnostic procedures.