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TaqMan™, RNA-to-CT™ TaqMan™ RNA-to-CT™ 1-Step Kit
Delivers consistent RNA target quantification for a wide variety of assays and is validated with Applied Biosystems™ comprehensive library of TaqMan™ Gene Expression assays.
Brand: TaqMan™, RNA-to-CT™ 4392653
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Description
The TaqMan™ RNA-to-CT™ One-Step Kit is recommended for a variety of applications including general gene expression studies, biomarker discovery, and microarray validation.
- Accurate across a wide dynamic range for dependable performance
- High sensitivity, enabling low copy number detection and precise quantification
- Validated with TaqMan™ Gene Expression Assays for easy experimental setup
- Consistent performance with a wide variety of targets, including AT-rich, GC-rich and long sequences
- Fewer pipetting steps means less hands-on-time and less chance for errors
Efficiency across a Broad Range of Input Concentrations
- Maximum flexibility in RNA template input quantity requires optimal real-time PCR efficiency across a broad range of template quantities
- The result is comparable to that of the TaqMan™ RNA-to-CT™ 2-Step Kit, and is a significant improvement in dynamic range and sensitivity over the TaqMan™ One-Step RT-PCR Master Mix Reagents.
High Sensitivity at Low Target Concentration
- The sensitivity of TaqMan™ RNA-to-CT™ One-Step Kit was validated using a synthetic RNA template for which copy number is precisely known
- Significant sampling error occurs when measuring low quantities of target, so statistical analysis is required for proper evaluation using multiple replicates
- Statistical analysis indicates high confidence of sample quantification based on a T-test, consistent with as few as 10 copies of target.
Order Info
Shipping Conditions: Wet Ice
Specifications
| Sufficient for 40 reactions at 50 μL reaction volume. Includes: • 1 x 1 mL tube of 2X master mix containing AmpliTaq Gold™ DNA Polymerase UP, dNTPs (including dUTP), Passive Reference 1, and optimized buffer components. Store at -15 to -25°C. • 1 x 50 μL tube of 40X RT enzyme mix containing ArrayScript™ UP Reverse Transcriptase, RNase Inhibitor. Store at -15° to -30°C. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). |
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| 1-step RT-qPCR | |
| Standard | |
| ROX (Pre-mixed) | |
| One-Step RT-PCR Kit | |
| 40 reactions | |
| Wet Ice | |
| Gene Expression | |
| RNA |
| Primer-probe | |
| AmpliTaq Gold DNA Polymerase | |
| 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode | |
| RNA-to-CT™, TaqMan™ | |
| UP (Ultra Pure) | |
| ArrayScript™ UP | |
| 40 Reactions | |
| 40 Reactions |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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